Purified Non-muscle Myosin Heavy Chain II-B Antibody (Previously Covance catalog# PRB-445P)

Pricing & Availability
Clone
Poly19099 (See other available formats)
Other Names
Myosin-10, NMMHC-B, myosin heavy chain 10, nonmuscle myosin heavy chain-B, nonmuscle myosin heavy chain IIB, cellular myosin heavy chain, type B type B
Previously
Covance Catalog# PRB-445P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
1 reviews
Product Citations
publications
Poly19099_Pure_ChainII-B_Antibody_1_111417
Western blot analysis of cell lysates from Hela (positive control), Daudi (low expression negative control) (Human) and UMR106 (Rat) using Non-muscle Myosin Heavy Chain II-B (Myosin-10) Rabbit primary antibody and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
  • Poly19099_Pure_ChainII-B_Antibody_1_111417
    Western blot analysis of cell lysates from Hela (positive control), Daudi (low expression negative control) (Human) and UMR106 (Rat) using Non-muscle Myosin Heavy Chain II-B (Myosin-10) Rabbit primary antibody and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
  • Poly19099_Pure_ChainII-B_Antibody_2_111417
    Immunofluorescence of HeLa cells with (A) Rabbit Isotype control (Cat. No. 910801) or (B-D) Non-muscle Myosin Heavy Chain II-B primary Antibody. Alexa Fluor® 594 (Red) Donkey anti-Rabbit IgG (Cat. No. 406418) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective. Exposure time (Seconds) for (A) is 1/30, and (B-D) is 1/60. Concentrations for (A, B) is 2 µg/ml, (C) is 1 µg/ml, (D) is 0.5 µg/ml.
Cat # Size Price Quantity Avail. Save
909902 25 µl $75
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909901 100 µl $245
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Description

Non-muscle Myosin Heavy Chain II-B is a member of the myosin superfamily. There are three isoforms of myosin II, called myosin IIA, myosin IIB and myosin IIC. These proteins are involved in cell motility, maintenance of cell shape, and cytokinesis. Each type of myosin II protein consists of two heavy chains and four light chains. The heavy chains each have two parts: a myosin head-like domain and a tail region. The head region interacts with actin, a protein that is important for cell movement and shape. The long tail region interacts with other proteins, including the tail regions of other myosin proteins.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, Rodent
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against the peptide SDVNETQPPQSE corresponding to the C-terminus of nonmuscle myosin heavy chain isoform B (MHC-B).
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC - Validated
IHC-P, IHC-F - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml (1:1000 - 1:2000). For immunocytochemistry, the suggested use of this reagent is 0.5 - 2.0 µg per ml (1:500 - 1:2000). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC) and immunofluorescence (IF).

*Predicted MW = 200 kD

Positive Control: human smooth muscle


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Ma X, et al. 2007. Mol Biol Cell. 18:2305. (IF)
  2. Golomb E, et al. 2004. J Biol Chem. 279:2800. (WB, IF)
  3. Song J, et al. 2001. Mol Cell Neurosci. 17:624.
  4. Kelley CA, et al. 1996. J Cell Biol. 134:675.
  5. Kelley CA, et al. 1995. J Biol Chem. 270:1395.
  6. Phillips CL, et al. 1995. J Muscl Res Cell Motil. 16:379.
  7. Skau CT, et al. 2015. PNAS. 112:2447. PubMed
  8. Wang H, et al. 2008. J. Cell Biol. 182: 1171. (ICC, WB) PubMed
  9. Hirota Y, et al. 2010. Development. 137: 3037. (IF) PubMed
Product Citations
  1. Li X, et al. 2015. Proc Natl Acad Sci U S A. 112:6080. PubMed
  2. Skau C, et al. 2015. Proc Natl Acad Sci U S A. 112: 2447 - 2456. PubMed
  3. Wang H, et al. 2008. J Cell Biol. 182:1171-1184. PubMed
  4. Hirota Y, et al. 2010. Development. 137:3037-3046. PubMed
  5. Loebel D, et al. 2016. Biol Open. 10.1242/bio.014415. PubMed
  6. Ramkumar N, et al. 2016. Nat Cell Biol. 18:1281-1291. PubMed
  7. S Tokuda, T Hirai, M Furuse 2016. PLoS One. 11:e0166904. PubMed
  8. Salomon J, et al. 2017. Nat Commun. 8:13998. PubMed
  9. Priya R, et al. 2017. Mol Biol Cell. 28(1):12-20. PubMed
  10. Trivedi N, et al. 2017. Nat Commun. 8:14484. PubMed
  11. Tokuda S, Hirai T, and Furuse M. 2017. PLoS One.. 10.1371/journal.pone.0182521. PubMed
  12. Nguyen-Ngoc K, et al. 2017. J Cell Sci. 10.1242/jcs.208546. PubMed
  13. Cartagena-Rivera A, Van Itallie C, and Anderson J. 2017. Nat Commun. . 10.1038/s41467-017-01145-8. PubMed
  14. Otterpohl K, et al. 2017. Physiol Rep.. 10.14814/phy2.13513. PubMed
  15. Sumigray KD 2018. Developmental cell. 45:183. PubMed
  16. Chopra A 2018. Developmental cell. 44:87. PubMed
  17. Van Itallie CM, et al. 2018. J Cell Sci. 131:. PubMed
  18. Fenix AM, et al. 2018. Elife. 7:. PubMed
Publication Library
RRID
AB_2749903 (BioLegend Cat. No. 909902)
AB_2565101 (BioLegend Cat. No. 909901)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Gene ID
4628 View all products for this Gene ID
UniProt
View information about Myosin-10 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 8    Revision Date: 08/05/2019

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