Purified Mouse IgG1, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MG1-45 (See other available formats)
Isotype
Mouse (BALB/c) IgG1, κ
Ave. Rating
1 reviews
Product Citations
publications
Cat # Size Price Quantity Avail. Save
401401 50 µg $40
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401402 500 µg $110
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Description

The isotype of MG1-45 immunoglobulin is mouse IgG1, κ. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested
WB, IP, ICC, IF, IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

The MG1-45 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 401404) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 401408) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Ozeki M, et al. 2008. J. Leukocyte Biol. 84:769. PubMed
  2. Kraetzel K, et al. 2008. Eur. Respair. J. 32:563. PubMed
  3. de Jong MA, et al. 2008. J Gen Virol. 89:2398. PubMed
  4. Romee R, et al. 2012 Blood. 120:4751. PubMed.
  5. Wong-Baeza I, et al. 2013. J. Immunol. 190:3216. PubMed.
Product Citations
  1. Jong M, et al. 2008. J Gen Virol. 89:2398. PubMed
  2. Shively M 2008. J Leukoc Biol. 84:769. PubMed
  3. Kraetzel K, et al. 2008. Eur Respir J. 32:563. PubMed
  4. Evesson F, et al. 2010. J Biol Chem. 285:28529. PubMed
  5. Ji Y, et al. 2012. J Biol Chem. 287:24378. PubMed
  6. Romee R, et al. 2012. Blood. 120:4751. PubMed
  7. Wong-Baeza I, et al. 2013. J Immunol. 190:3216. PubMed
  8. Murayama M, et al. 2015. Nat Commun. 6: 8483. PubMed
  9. Barzilai S, et al. 2016. J Leukoc Biol. 99: 1045 - 1055. PubMed
  10. Matsuba S, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01538. PubMed
  11. Espinosa-Díez C, et al. 2018. Redox Biol. 14:88. PubMed
  12. Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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