Purified anti-XBP-1s Antibody

Pricing & Availability
Clone
143F (See other available formats)
Other Names
X-box binding protein 1
Isotype
Mouse IgG2a
Ave. Rating
3 reviews
Product Citations
publications
a-143F_Xbp1s_111810
Extracts of untreated HepG2 cells (lane 1) and extracts of HepG2 cells treated with 300 nM thapsigargin (TG) for 16 hours (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Xbp-1s antibody (clone 143F). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • a-143F_Xbp1s_111810
    Extracts of untreated HepG2 cells (lane 1) and extracts of HepG2 cells treated with 300 nM thapsigargin (TG) for 16 hours (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Xbp-1s antibody (clone 143F). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • b-143F_PURE_Xbp1s_Antibody_2_070620
    Chromatin Immunoprecipitations (ChIP) were performed using fixed and sonicated chromatin samples from 293T cells treated with tunicamycin (2.0 µg/mL, 8 hr). ChIP was performed with 10.0 µg of chromatin and 2.0 µg of purified anti- XBP-1s antibody (clone 143F) or equal amount of Go-ChIP-Grade™ purified mouse IgG2a, κ isotype ctrl antibody (Cat. No. 401505). The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for the human DNAJB9 exon 1 region and the human α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as percentage of the total amount of input chromatin, which is equivalent to 100%
Cat # Size Price Quantity Avail. Save
647501 25 µg $105
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647502 100 µg $250
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Description

XBP-1 is a transcription factor containing a bZIP domain. It was first identified because of its ability to bind X-box, a conserved transcriptional element in the promoter of human HLA DR gene. XBP-1 has multiple functions. It controls MHC class II gene regulation and is also essential for differentiation of plasma cells. XBP-1 upregulates as part of the ER stress response, also known as the unfolded protein response. Unspliced XBP-1 is 261 amino acids and migrates on the SDS-PAGE around 33 kD; spliced XBP-1, recognized by clone 143F, is 371 amino acids and migrates around 55 kD.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
XBP-1s recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ChIP - Validated
ICC, IHC-P - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 to 2.0 µg per mL. For ChIP application, use 2 µg of antibody per 10 µg of chromatin per IP. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence14 and immunohistochemical staining of PFA-fixed paraffin-embedded sections1,14.

Additional Product Notes

Using this antibody at a ratio higher than 1:5 (µg antibody per µg of chromatin) will increase background.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsang KY, et al. 2007. PLOS Biol. 5:568. (IHC) PubMed
  2. Farhan H, et al. 2008. EMBO J. 27:2043 (WB) PubMed
  3. Farhan H, et al. 2010. J. Cell Biol. 189:997. PubMed
  4. Feng-Jin G, et al. 2010. Cell Signal. [Epub ahead of print]
  5. Wang L, et al. 2011. Hum Mol Genet. PubMed
  6. Li J, et al. 2011. J. Biol Chem. 286:4912. PubMed
  7. Wang L, et al. 2011. Hum Mol Genet. 20:1008. PubMed
  8. Liu Y, et al. 2011. J Biol Chem. 286:13161. PubMed
  9. Shirley CM, et al. 2011 Blood. 117:6297. PubMed
  10. Vidal RL., et al. 2012. Hum Mol Genet. PubMed
  11. Byrd AE, et al. 2012. J Cell Biol. 196:689. PubMed
  12. Dickie LJ, et al. 2012. Ann rheum Dis. 71:2035. PubMed
  13. Bonetti P, et al. 2013. Blood. 122:2233. PubMed
  14. Maestre L, et al. 2009. Haematologica. 94:419. (IF, IHC)
  15. Rodriguez M, et al. 2014. J Biol Chem. 289:22942. PubMed
Product Citations
  1. Farhan H, et al. 2010. J Cell Biol. 189:997. PubMed
  2. Shirley C, et al. 2011. Blood. 117:6297. PubMed
  3. Liu Y, et al. 2011. J Biol Chem. 286:13161. PubMed
  4. Li J, et al. 2011. J Biol Chem. 286:4912. PubMed
  5. Wang L, et al. 2011. Hum Mol Genet. 1.533333333. PubMed
  6. Byrd A, et al. 2012. J Cell Biol. 196:689. PubMed
  7. Vidal R, et al. 2012. Hum Mol Genet. 2.434027778. PubMed
  8. Dickie L, et al. 2012. Ann Rheum Dis. 71:2035. PubMed
  9. Cai X, et al. 2015. Exp Cell Res. Available online 29 August 2015. PubMed
  10. Li X, et al. 2018. Sci Rep. 7:2013. PubMed
  11. Talty A, et al. 2019. Cell Death Dis. 10:622. PubMed
  12. Li J et al. 2018. Cell chemical biology. 25(11):1350-1358 . PubMed
RRID
AB_2241744 (BioLegend Cat. No. 647501)
AB_2241743 (BioLegend Cat. No. 647502)

Antigen Details

Distribution

Ubiquitously expressed.

Function
Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor.
Biology Area
Cell Biology
Antigen References

1. Liou HC, et al. 1990. Science 247:1581.
2. Ponath PD, et al. 1993. J. Biol. Chem.268:17074.
3. Takahashi S, et al. 2002. Prostate 50:154.

Gene ID
7494 View all products for this Gene ID
UniProt
View information about XBP-1s on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/06/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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