Purified anti-XBP-1s Antibody

Pricing & Availability
Clone
Poly6195 (See other available formats)
Other Names
X-box binding protein 1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
poly6195_PURE_XBP-1s_Antibody_A_WB_062116
Panel A: Western blot analysis of extracts from HepG2 untreated (lane 1), HepG2 treated with 300 nM of thapsigargin overnight (lane 2), mouse liver (lane 3) and mouse brain (lane 4) using anti-XBP-1s antibody (Poly6195).
  • poly6195_PURE_XBP-1s_Antibody_A_WB_062116
    Panel A: Western blot analysis of extracts from HepG2 untreated (lane 1), HepG2 treated with 300 nM of thapsigargin overnight (lane 2), mouse liver (lane 3) and mouse brain (lane 4) using anti-XBP-1s antibody (Poly6195).
  • poly6195_PURE_XBP-1s_Antibody_B_WB_062116
    Panel B: Western blot analysis of extracts from Raw264.7 untreated (lane 1) and Raw264.7 cells treated with 300 nM of thapsigargin overnight (lane 2) using anti-XBP-1s antibody (Poly6195).
Cat # Size Price Quantity Avail. Save
619502 200 µl (20 Western blots) $225
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Clones 143F and 9D11A43 are available as alternatives.
Description

Xbp-1 (X-box binding protein 1) is a ubiquitously expressed 29 kD member of the CREB/ATF transcription factor family, and a mammalian homologue of the yeast transcription factor Hac1. Xbp-1 regulates MHC class II genes by binding to the X-box promoter motif and can also bind to the enhancer in T cell leukemia virus type I promoter to increase viral protein expression by binding the viral transactivator. This transcription factor is essential for hepatocyte differentiation and can be upregulated by ER stress, IL-6, and IL-4. The PAX5 transcription factor downregulates Xbp-1; downregulation has been shown to correlate with tumor progression in prostate cancer. The Poly6195 antibody recognizes the C-terminal region of human and rat Xbp-1 and has been shown to be useful for Western blotting.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, and weak reactive with Mouse; recognizes the 54 kD form
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
ChIP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation8.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Hou J. 2005. Leuk Res 29:1059. PubMed
  2. Thuerauf DJ, et al. 2006. Circ Res.99:275. (WB) PubMed
  3. Kim KM, et al. 2007. Circ Res. 101:919. (WB) PubMed
  4. Zhou X, et al. 2007. Leuk Res. 31:1259 PubMed
  5. Jiang H, et al. 2007. Int J Hematol. 86:429 PubMed
  6. Romero-Ramirez L. 2009. Transl Oncol. 2:31 PubMed
  7. Pattison JS, et al. 2008. Circulation. 117:2743. (WB) PubMed
  8. Hsiao JR, et al. 2009 Cancer Res 69:4461 (ChIP) PubMed
  9. Guan L, et al. 2009, Apoptosis. 14:218. PubMed
  10. Scriven P, et al. 2009. Br J Cancer. 101:1692 PubMed
  11. Yu J, et al. 2009. Int J Biochem Cell Biol. 41:1739. PubMed
  12. Coope A, et al. 2012. J. Biol Chem. 287:15580. PubMed
  13. Kawabata S, et al. 2012. Cell Death Dis. 3:e353. PubMed
  14. Wang Y, et al. 2012. Cancer Res. 72:5396. PubMed
  15. Chang PJ, et al. 2014. J Virol. 88:10092. PubMed
  16. Yang W, et al. 2014. J Neurol Sci. 344:129. PubMed
  17. Deegan S, et al. 2015. Biochem Biophys Res Commun. 456:305. PubMed
  18. Sheng X, et al. 2015. EMBO Mol Med. 11:788. PubMed
  19. Vial G, et al. 2015. Diabetes. 64:2254. PubMed
  20. Bright MD, et al. 2015. Mol Cell Biol. 35:2186. PubMed
Product Citations
  1. Thuerauf D, et al. 2006. Circ Res . 99:275. PubMed
  2. Kim K, et al. 2007. Circ Res. 101:919. PubMed
  3. Pattison J, et al. 2008. Circulation. 117:2743. PubMed
  4. Coope A, et al. 2012. J Biol Chem. 287:15580. PubMed
  5. Kawabata S, et al. 2012. Cell Death Dis. 3:e353. PubMed
  6. Chang P, et al. 2014. J Virol. 88:10092. PubMed
  7. Yang W, et al. 2014. J Neurol Sci. 344:129. PubMed
  8. Deegan S, et al. 2015. Biochem Biophys Res Commun. 456:305. PubMed
  9. Sheng X, et al. 2015. EMBO Mol Med. 11:788. PubMed
  10. Vial G, et al. 2015. Diabetes. 64:2254. PubMed
  11. Bright M, et al. 2015. Mol Cell Biol. 35:2186. PubMed
  12. Iwao C, Shidoji Y 2015. PLoS One. 10: 0132761. PubMed
  13. Gupta A, et al. 2015. Sci Rep. 5: 18304. PubMed
  14. Hassan H, et al. 2016. J Am Soc Nephrol. 27: 1055-1065. PubMed
  15. Cho Y, et al. 2016. Exp Mol Med. 48: e226. PubMed
  16. Gupta A, et al. 2016. Oncogene. 10.1038/onc.2016.121. PubMed
  17. Ren Z, et al. 2017. Biochim Biophys Acta. 10.1016/j.bbadis.2017.07.035. PubMed
  18. Guo J, et al. 2018. Mol Neurodegener. 13:4. PubMed
  19. Hossain MM, et al. 2018. Oncotarget. 9:11707. PubMed
  20. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  21. Xie H, et al. 2018. J Clin Invest. 14:34. PubMed
  22. Estornes Y, et al. 2018. Cell Death Dis. 5. PubMed
  23. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  24. Kim S, et al. 2018. Mol Cells. 41:964. PubMed
  25. Chae U, et al. 2019. Oncogenesis. 8:12. PubMed
  26. Shi Z, et al. 2019. Sci Rep. 9:3210. PubMed
  27. Tsuchiya Y, et al. 2018. J Cell Biol. 217:1287. PubMed
  28. Blackwood EA, et al. 2019. Nat Commun. 10:187. PubMed
  29. Cai Y, et al. 2019. Cell Discov. 5:7. PubMed
  30. Xu S, et al. 2019. J Neuroinflammation. 16:35. PubMed
Publication Library
RRID
AB_315908 (BioLegend Cat. No. 619502)

Antigen Details

Structure
Member of the CREB/ATF transcription factor family, mammalian homologue of the yeast transcription factor Hac1. Predicted molecular weight approximately 30 kD and 54 kD (splicing variant)
Distribution

Ubiquitously expressed

Function
Transcription factor regulating MHC class II genes by binding to X-box promoter motif. Can also bind to enhancer in T cell leukemia virus type I promoter and enhance viral protein expression by acting as DNA binding partner of a viral transactivator.
Biology Area
Cell Biology, Immunology, Transcription Factors
Antigen References

1. Liou HC, et al. 1990. Science 247:1581.
2. Ponath PD, et al. 1993. J. Biol. Chem. 268:17074.
3. Takahashi S, et al. 2002. Prostate 50:154.

Regulation
Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor
Gene ID
7494 View all products for this Gene ID
UniProt
View information about XBP-1s on UniProt.org

Related FAQs

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Go To Top Version: 6    Revision Date: 06/21/2016

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