- Poly6195 (See other available formats)
- Other Names
- X-box binding protein 1
- Rabbit Polyclonal IgG
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- Product Citations
|Cat #||Size||Price||Quantity Avail.||Save|
|619502||200 µl (20 Western blots)||$225|
Xbp-1 (X-box binding protein 1) is a ubiquitously expressed 29 kD member of the CREB/ATF transcription factor family, and a mammalian homologue of the yeast transcription factor Hac1. Xbp-1 regulates MHC class II genes by binding to the X-box promoter motif and can also bind to the enhancer in T cell leukemia virus type I promoter to increase viral protein expression by binding the viral transactivator. This transcription factor is essential for hepatocyte differentiation and can be upregulated by ER stress, IL-6, and IL-4. The PAX5 transcription factor downregulates Xbp-1; downregulation has been shown to correlate with tumor progression in prostate cancer. The Poly6195 antibody recognizes the C-terminal region of human and rat Xbp-1 and has been shown to be useful for Western blotting.Product Details
- Human, Rat, and weak reactive with Mouse; recognizes the 54 kD form
- Antibody Type
- Host Species
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
- The antibody was purified by antigen-affinity chromatography.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Upon receipt, store frozen at -20°C.
WB - Quality tested
ChIP - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation8.
This product may contain other non-IgG subtypes.
(PubMed link indicates BioLegend citation)
- Hou J. 2005. Leuk Res 29:1059. PubMed
- Thuerauf DJ, et al. 2006. Circ Res.99:275. (WB) PubMed
- Kim KM, et al. 2007. Circ Res. 101:919. (WB) PubMed
- Zhou X, et al. 2007. Leuk Res. 31:1259 PubMed
- Jiang H, et al. 2007. Int J Hematol. 86:429 PubMed
- Romero-Ramirez L. 2009. Transl Oncol. 2:31 PubMed
- Pattison JS, et al. 2008. Circulation. 117:2743. (WB) PubMed
- Hsiao JR, et al. 2009 Cancer Res 69:4461 (ChIP) PubMed
- Guan L, et al. 2009, Apoptosis. 14:218. PubMed
- Scriven P, et al. 2009. Br J Cancer. 101:1692 PubMed
- Yu J, et al. 2009. Int J Biochem Cell Biol. 41:1739. PubMed
- Coope A, et al. 2012. J. Biol Chem. 287:15580. PubMed
- Kawabata S, et al. 2012. Cell Death Dis. 3:e353. PubMed
- Wang Y, et al. 2012. Cancer Res. 72:5396. PubMed
- Chang PJ, et al. 2014. J Virol. 88:10092. PubMed
- Yang W, et al. 2014. J Neurol Sci. 344:129. PubMed
- Deegan S, et al. 2015. Biochem Biophys Res Commun. 456:305. PubMed
- Sheng X, et al. 2015. EMBO Mol Med. 11:788. PubMed
- Vial G, et al. 2015. Diabetes. 64:2254. PubMed
- Bright MD, et al. 2015. Mol Cell Biol. 35:2186. PubMed
- Product Citations
AB_315908 (BioLegend Cat. No. 619502)
- Member of the CREB/ATF transcription factor family, mammalian homologue of the yeast transcription factor Hac1. Predicted molecular weight approximately 30 kD and 54 kD (splicing variant)
- Transcription factor regulating MHC class II genes by binding to X-box promoter motif. Can also bind to enhancer in T cell leukemia virus type I promoter and enhance viral protein expression by acting as DNA binding partner of a viral transactivator.
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Antigen References
1. Liou HC, et al. 1990. Science 247:1581.
2. Ponath PD, et al. 1993. J. Biol. Chem. 268:17074.
3. Takahashi S, et al. 2002. Prostate 50:154.
- Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor
- Gene ID
- 7494 View all products for this Gene ID
- View information about XBP-1s on UniProt.org
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