Purified anti-WAVE1 Antibody (Previously Covance catalog# MMS-5208)

Pricing & Availability
Clone
K91/36 (See other available formats)
Other Names
Wiskott-Aldrich syndrome protein family member 1, protein WAVE-1, WASP family protein member 1, homology of dictyostelium scar 1, verprolin homology domain-containing protein 1
Previously
Covance Catalog# MMS-5208
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
K91slash36_Purified_WAVE1_Antibody_1_020918
Western blot of purified anti-WAVE1 antibody (clone K91/36). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • K91slash36_Purified_WAVE1_Antibody_1_020918
    Western blot of purified anti-WAVE1 antibody (clone K91/36). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • K91slash36_Purified_WAVE1_Antibody_2_020918
    Western blot of purified anti-WAVE1 antibody (clone K91/36). Lane 1: Molecular weight marker; Lane 2: 15 µg of rat brain membrane lysate; Lan 3: 15 µg of mouse brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • K91slash36_Purified_WAVE1_Antibody_3_020918
    IHC staining of purified anti-WAVE1 antibody (clone K91/36) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • K91slash36_Purified_WAVE1_Antibody_4_020918
    IHC staining of purified anti-WAVE1 antibody (clone K91/36) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • K91slash36_Purified_WAVE1_Antibody_5_020918
    IHC staining of purified anti-WAVE1 antibody (clone K91/36) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
817901 100 µl $140.00
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Description

Organ morphogenesis can occur through cellular response to extracellular signals that alter actin dynamics. Wiskott-Aldrich syndrome protein-family verprolin homologous protein 1 (WAVE1) is an actin-interacting protein involved in brain morphogenesis. WAVE1 interacts with Arp (actin-related proteins) 2/3 to regulate the cytoskeleton. WAVE proteins mediate lamellipodia formation and are critical for normal CNS myelination. WAVE1 can be phosphorylated by Cdk5 to downregulate Arp2/3 complex-dependent actin polymerization.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide corresponding to amino acids 477-498 of human Wave1/Wasp family 1 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10.0 µg per ml For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and paraffin-embedded immunohistochemical staining (IHC-P).

RRID
AB_2564815 (BioLegend Cat. No. 817901)

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Go To Top Version: 3    Revision Date: 02/09/2018

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