Purified anti-VSV-G Epitope Tag Antibody (Previously Covance catalog# PRB-192P)

Pricing & Availability
Clone
Poly29039 (See other available formats)
Other Names
Vesicular stomatitis virus glycoprotein tag, VSV epitope tag, VSV tag, VSV-G epitope tag, VSVG, vsvg tag, YTDIEMNRLGK
Previously
Covance Catalog# PRB-192P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
2 reviews
Product Citations
publications
Poly29039_Purified_VSV-G_Epitope_Antibody_WB_1_123118.png
0.1 µL of Recombinant Posi-Tag Epitope Tag Protein (Cat. No. 931301) was resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.0033 µg/mL (1:30000 dilution) of Purified anti-VSV-G Epitope Tag Antibody, clone Poly29039, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Donkey anti- rabbit IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Lane M: Molecular Weight marker.
  • Poly29039_Purified_VSV-G_Epitope_Antibody_WB_1_123118.png
    0.1 µL of Recombinant Posi-Tag Epitope Tag Protein (Cat. No. 931301) was resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.0033 µg/mL (1:30000 dilution) of Purified anti-VSV-G Epitope Tag Antibody, clone Poly29039, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Donkey anti- rabbit IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
903901 100 µl $281
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Description

The VSV-G epitope tag is used to label a protein of interest so that the tagged protein can be analyzed and visualized. Antibodies against VSV-G epitope tag recognize tagged proteins and allow for specific detection in several immunochemical assays such as western blotting, ELISAs and immunostaining techniques.

Product Details
Technical data sheet

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Polyclonal VSV-G antisera was raised against the peptide for VSV-G (YTDIEMNRLGK) conjugated to KLH.
Formulation
Phosphate-buffered solution with 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, ICC, ELISA, Purification

Recommended Usage

The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. BioLegend antibodies may not be resold or modified for resale without prior written approval.

WB: 1:1,000-30,000*
IHC: 1:100-400
ELISA: 1:100-500 (coating), 1:1,000-30,000 (primary)

Application Notes

This antibody is effective in immunoblotting, immunocytochemistry and ELISA.

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

The antisera was purified via affinity chromatography using the peptide immobilized on solid support.

This product may contain other non-IgG subtypes.

Posi-Tag is a ~45 kD recombinant protein that contains 12 different tags. It has a predicted weight of 45 kD and is intended for use as a positive control in western blot applications. The lysate contains the commonly used epitope tags HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, and FLAG™ tag.

Application References

(PubMed link indicates BioLegend citation)
  1. Kang S, et al. 2006. Proc Natl Acad Sci USA 103:1289. (WB) PubMed
Product Citations
  1. Kang S, et al. 2006. Proc Natl Acad Sci U S A. 103:1289-1294. PubMed
Publication Library
RRID
AB_2565035 (BioLegend Cat. No. 903901)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about VSV-G on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 01/25/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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