Purified anti-Vimentin Antibody

Pricing & Availability
Clone
W16220A (See other available formats)
Other Names
Vim, CTRCT30, HEL 113, Epididymis Luminal Protein 113
Isotype
Rat IgG2a, κ
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Product Citations
publications
W16220A_PURE_Vimentin_Antibody_1_091417
Total cell lysate (15 µg protein) from A20 (negative control), EL4, NIH3T3 and HeLa cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1: 500 (1 µg/mL) diluted purified anti-Vimentin antibody (clone W16220A). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • W16220A_PURE_Vimentin_Antibody_1_091417
    Total cell lysate (15 µg protein) from A20 (negative control), EL4, NIH3T3 and HeLa cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1: 500 (1 µg/mL) diluted purified anti-Vimentin antibody (clone W16220A). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • W16220A_PURE_Vimentin_Antibody_2_031819
    NIH3T3 cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:1000 (0.5 µg/mL) anti-Vimentin (clone W16220A) overnight at 4 degrees followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG incubation for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective. For negative control and titration data, refer to the supplemental data.
Cat # Size Price Quantity Avail. Save
699301 25 µg $85
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699302 100 µg $215
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Description

Vimentin are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is a widely expressed and highly conserved 54 kD protein that is constitutively expressed in mesenchymal cells, endothelial cells lining blood vessels, renal tubular cells, macrophages, neutrophils, fibroblasts, and leukocytes1,2. Vimentin is used as a marker of mesenchymal cells to distinguish them from epithelial cells3. Increased vimentin expression is frequently used as an EMT marker in cancer4. Autoantibodies to vimentin are commonly found in patients with autoimmune diseases such as Lupus5 and rheumatoid arthritis6, and also found after transplantation7.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial mouse Vimentin (224-446 a.a.) recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1: 500-1:5000 diluted (0.1 – 1 µg/mL). For immunocytochemistry, the suggested use of this reagent is 1:250-1:2500 diluted (0.2 – 2 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody does not cross-react with human (in-house tested).

May be weakly reactive with human Vimentin by WB.

RRID
AB_2716136 (BioLegend Cat. No. 699301)
AB_2716137 (BioLegend Cat. No. 699302)

Antigen Details

Structure
466 amino acids with a predicted molecular weight of 54 kD.
Distribution

Cytoplasm.

Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Interaction
HCV core protein, LGSN, SYNM, PLEC, SLC6A4, STK33, LARP6, RAB8B, TOR1A, TOR1AIP1, and BCAS3.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Kidd ME, et al. 2014. Am J Respir Cell Mol Biol. 50:1.
2. Fuchs E, et al. 1994. Annu Rev Biochem. 63:345.
3. Zeisberg M, et al. 2009. J Clin Invest. 119:1429.
4. Scanlon CS, et al. 2013. J Dent Res. 92:114.
5. Thebault S, et al. 2002. J Immunol. 169:4046.
6. Vossenaar ER, et al. 2004. Arthritis Res Ther. 6:R142.
7. Rose ML. 2013. Hum Immunol. 74:1459.

Gene ID
22352 View all products for this Gene ID
UniProt
View information about Vimentin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 09/18/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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