Purified anti-VEGF-165 Antibody

Pricing & Availability
Clone
L308D10 (See other available formats)
Other Names
Vascular Endothelial Growth Factor A, Vascular Permeability Factor, Vascular Endothelial Growth Factor, VEGF-A, VPF, MVCD1
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
L308D10_Purified_VEGF-165_Antibody_IP_092914
The VEGF-165 antibody (L308D10) was tested with both immunoprecipitation and Western blot analysis. The supernatant of cells over-expressing VEGF-165 was tested. Lane 1 was immunoprecipitated with mouse IgG control antibody and lane 2 was immunoprecipitated with VEGF-165 antibody (L308D10). Western blot analysis was performed using rabbit anti-human VEGF antibody (poly6275).
  • L308D10_Purified_VEGF-165_Antibody_IP_092914
    The VEGF-165 antibody (L308D10) was tested with both immunoprecipitation and Western blot analysis. The supernatant of cells over-expressing VEGF-165 was tested. Lane 1 was immunoprecipitated with mouse IgG control antibody and lane 2 was immunoprecipitated with VEGF-165 antibody (L308D10). Western blot analysis was performed using rabbit anti-human VEGF antibody (poly6275).
Cat # Size Price Quantity Avail. Save
662702 100 µg $105
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Description

VEGF-A belongs to the VEGF family, which consists of VEGF-A, VEGF-B, VEGF-C, VEGF-D, and PlGF (placental growth factor). VEGF-A is alternatively spliced to generate several variants such as VEGF-121, VEGF-145, VEGF-165, and VEGF-189. VEGF-165 is predominant and responsible for the potency of VEGF-A. VEGF-A specifically acts on endothelial cells and plays important roles in endothelial cell proliferation, angiogenesis, and vasculogenesis. It is highly expressed in tumors and is one of the most important targets for cancer therapy.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human VEGF-165
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IP - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunoprecipitation. This antibody can be used at 2 - 10 µg/mL for immunoprecipitation. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564372 (BioLegend Cat. No. 662702)

Antigen Details

Structure
232 amino acids with predicted molecular weight of approximately 27 kD.
Distribution
Widely expressed.
Function
Endothelial cell proliferation, angiogenesis, and vasculogenesis.
Interaction
Binds to heparin sulfate, heparin, and neuropilin-1. Interacts with vascular endothelial cells, monocytes, and macrophages, which express VEGFR1; this induces proliferation of endothelial cells and stimulates the migration of monocytes and macrophages.
Ligand/Receptor
FLT1/VEGFR1 and KDR/VEGFR2.
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Signal Transduction
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Conn G, et al. 1990. Proc. Natl. Acad. Sci. USA 87:1323.
2. Gerber H, et al. 2002. Nature 417:954.
3. Shibuya M, et al. 2006. J. Biochem. Mol. Biol. 39:469.
4. Reddy K, et al. 2008. Angiogenesis 11:257.
5. Monaghan-Benson E, et al. 2010. Am. J. Pathol. 177:2091.
6. Koch S and Claesson-Welsh L. 2012. Cold Spring Harb. Perspect. Med. 2:a006502.
7. Cao Y. 2014. Nat. Rev. Endocrinol. 10:530.
8. Lui KO. 2014. Curr. Stem Cell Res. Ther. 9:396.

Gene ID
7422 View all products for this Gene ID
UniProt
View information about VEGF-A on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 10/17/2016

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