Purified anti-VDAC1 Antibody (Previously Covance catalog# MMS-5205)

Pricing & Availability
Clone
N152B/23 (See other available formats)
Other Names
Voltage-dependent anion-selective channel protein 1, porin 31HL, porin 31HM, plasmalemmal porin, outer mitochondrial membrane protein porin 1, PORIN, VDAC-1
Previously
Covance Catalog# MMS-5205
Isotype
Mouse IgG2a
Ave. Rating
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Product Citations
publications
N152Bslash23_Purified_VDAC1_Antibody_1_WB_030415
Western blot of anti-VDAC1 antibody (clone N152B/23). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N152Bslash23_Purified_VDAC1_Antibody_1_WB_030415
    Western blot of anti-VDAC1 antibody (clone N152B/23). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N152Bslash23_Purified_VDAC1_Antibody_2_IHC_030415
    IHC staining of anti-VDAC1 antibody (clone N152B/23) on formalin-fixed paraffin-embedded human placenta tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • N152Bslash23_Purified_VDAC1_Antibody_3_IHC_030415
    IHC staining of anti-VDAC1 antibody (clone N152B/23) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • N152Bslash23_Purified_VDAC1_Antibody_4_IHC_030415
    IHC staining of anti-VDAC1 antibody (clone N152B/23) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • N152Bslash23_Purified_VDAC1_Antibody_5_IHC_030415
    IHC staining of anti-VDAC1 antibody (clone N152B/23) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
Cat # Size Price Quantity Avail. Save
820702 25 µL $70
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820701 100 µL $140
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Description

Voltage-dependent anion-selective channel protein 1 (VDAC1, also known as outer mitochondrial membrane protein porin 1, porin 31HL, and plasmalemmal porin) is a voltage-dependent anion channel and is the main calcium ion transport channel and plays a role in regulating the citric acid cycle and ROS generation. VDAC1 is implicated in cancer and neurodegenerative diseases through its ability to elevate ROS production.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-283 (full-length) of human VDAC1.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry of formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

Product Citations
  1. Enming Zhang et al. 2018. Cell metabolism. 29(1):64-77 . PubMed
RRID
AB_2750531 (BioLegend Cat. No. 820702)
AB_2564843 (BioLegend Cat. No. 820701)

Antigen Details

Structure
VDAC1 is a 283 amino acid protein with a molecular weight of 30 kD.
Biology Area
Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
Molecular Family
Mitochondrial Markers
Gene ID
7416 View all products for this Gene ID
UniProt
View information about VDAC1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/24/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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