Purified anti-Ubiquitin Antibody

Pricing & Availability
Clone
P4D1 (See other available formats)
Isotype
Mouse IgG1,κ
Ave. Rating
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Product Citations
publications
P4D1 072709
Mouse recombinant ubiquitin was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Ubiquitin antibody, clone P4D1. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
  • P4D1 072709
    Mouse recombinant ubiquitin was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Ubiquitin antibody, clone P4D1. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
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646301 25 µg $105
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646302 100 µg $225
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Description

Ubiquitin is a highly-conserved small protein expressed in all eukaryotes. It is involved in a post-translation modification of proteins called ubiquitination of proteins by the covalent attachment (via an isopeptide bond) of one or more than one ubiquitin monomers. Ubiqutination controls the protein degradation, stability and intracellular localization of many proteins.

Product Details
Technical data sheet

Product Details

Reactivity
All species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Denatured glutaraldehyde cross-linked ubiquitin IgG complex
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.12 to 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins. This clone is equivalent to Clone P4G7. The ubiquitin protein is extremley well conserved and thus the antibody has extensive species cross-reactivity from yeast to human. This antibody was specifically developed to detect ubiquitin and ubiquitin-substrate conjugates by immunoblotting. Because the antibody recognizes a denatured epitope, it is known to not work in immunoprecipitation or immunostaining applications.

Application References

(PubMed link indicates BioLegend citation)
  1. Magnaudeix A, et al. 2013. Neurobiol Aging. 34:770. PubMed.
Product Citations
  1. Schreiner J, et al. 2013. Neurobiol Aging. 34:770. PubMed
  2. Deguise M, et al. 2016. Sci Rep. 6:28846. PubMed
  3. Bermudez-Brito M, Faas M, Vos P 2016. Nature. 10.1038/nature18943. PubMed
  4. Shi Y, et al. 2016. Cell Death Differ. 10.1038/cdd.2016.110. PubMed
Publication Library
RRID
AB_1659270 (BioLegend Cat. No. 646301)
AB_1659269 (BioLegend Cat. No. 646302)

Antigen Details

Structure
Consists of 76 amino acids and has a molecular mass of about 8.5 kd. Highly conserved among all eukaryotic species.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
Molecular Family
Autophagosome Markers
Antigen References

1. Kahana, et al. 1999. Mol. Cell Biol 19:6608
2. Ciechanover A, et al. 1998. EMBO J 17:7151
3. Xu P, et al. 2008. Analytical Chemistry 80:3438

Gene ID
6233 View all products for this Gene ID
UniProt
View information about Ubiquitin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/20/2016

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