Purified anti-Transthyretin, aggregated Antibody

Pricing & Availability
Clone
TA5F4 (See other available formats)
Other Names
PALB, ATTR, Prealbumin, TBPA, TTR
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TA5F4_PURE_Transthyretin_CAPTUREELISA_010317_resized
Congo red (A) and purified anti-Transthyretin, aggregated antibody (clone TA5F4) (B) staining of Transtheyretin amyloid deposits in formalin-fixed paraffin-embedded heart tissue of a patient with familial amyloidotic polyneuropathy (FAP). Following antigen retrieval using Antigen Retrieval Citra Plus (BioGenex), the tissue was incubated with the primary antibody at 3 µg/mL overnight at 4°C. Horse radish peroxidase conjugated anti-mouse IgG was used as the detection system.
  • TA5F4_PURE_Transthyretin_CAPTUREELISA_010317_resized
    Congo red (A) and purified anti-Transthyretin, aggregated antibody (clone TA5F4) (B) staining of Transtheyretin amyloid deposits in formalin-fixed paraffin-embedded heart tissue of a patient with familial amyloidotic polyneuropathy (FAP). Following antigen retrieval using Antigen Retrieval Citra Plus (BioGenex), the tissue was incubated with the primary antibody at 3 µg/mL overnight at 4°C. Horse radish peroxidase conjugated anti-mouse IgG was used as the detection system.
  • TA5F4_PURE_Transthyretin_IHC_1_010317
  • TA5F4_PURE_Transthyretin_IHC_2_010317
    Congo red (A) and purified anti-Transthyretin, aggregated antibody (clone TA5F4) (B) staining of Transtheyretin amyloid deposits in formalin-fixed paraffin-embedded heart tissue of a patient with familial amyloidotic polyneuropathy (FAP). Following antigen retrieval using Antigen Retrieval Citra Plus (BioGenex), the tissue was incubated with the primary antibody at 3 µg/mL overnight at 4°C. Horse radish peroxidase conjugated anti-mouse IgG was used as the detection system.-------
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848101 25 µg $95
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848102 100 µg $245
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Description

Transthyretin (TTR) is a carrier protein capable of transporting thyroid hormones in the plasma and cerebrospinal fluid, and retinol (vitamin A) in the plasma. TTR can form aberrant, insoluble amyloid fibrils that primarily deposit in a patient's heart, peripheral nerves, or both. The wild-type protein is associated with senile systemic amyloidosis (SSA) and is typically diagnosed in men over 70-years-old. Single or double mutations in TTR are primarily associated with familial amyloidotic polyneuropathy (FAP) or familial amyloid cardiopathy (FAC) which affects younger patients, often in their 30s. Clinical symptoms of TTR amyloidosis have historically been attributed to fibrils, but recent experimental evidence indicates that soluble TTR aggregates are also pathogenic.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
A 1:1 mixture of wild-type Transthyretin fibrils and soluble aggregates of Y78F TTR.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IHC-P - Validated
Direct ELISA - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration of 2.0 µg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration of is 1.0 - 5.0 µg/ml is recommmended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone TA5F4 recognizes an epitope on aggregated transthyretin that encompasses mid- and N-terminal regions.

Application References

(PubMed link indicates BioLegend citation)
  1. Phay M, et al. 2014. Rejuvenation Res. 17(2):97. (Direct ELISA, Capture ELISA, IHC) PubMed
RRID
AB_2632815 (BioLegend Cat. No. 848101)
AB_2632816 (BioLegend Cat. No. 848102)

Antigen Details

Structure
Transthyretin (TTR) is a β-sheet rich homotetrameric protein that has a molecular mass of 55 kD. Each monomer consists of 127 amino acids.
Distribution
Primarily extracellular in blood and cerebrospinal fluid.
Function
The normal function of TTR is to transport thyroxine and retinol-binding protein in the blood and cerebrospinal fluid.
Interaction
Perlecan, receptor for advanced glycation end product (RAGE).
Ligand Receptor
Thyroxine, retinol-binding protein bound to retinol.
Cell Sources
Liver, choroid plexus epithelium, retina pigment epithelium.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Carrier Proteins
Antigen References

1. Plante-Bordeneuve V, Said G. 2011. Lancet Neurol. 10:1086. PubMed
2. Pinney JH, et al. 2013. J. Am. Heart Assoc. 2:e000098. PubMed
3. Hawkins PN, et al. 2015. Ann Med. 47(8):625. PubMed

Gene ID
7276 View all products for this Gene ID
UniProt
View information about Transthyretin, aggregated on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 12/28/2016

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