Purified anti-TIF1β (KAP-1, TRIM28) Antibody

Pricing & Availability
Clone
20A1 (See other available formats)
Other Names
Transcription intermediary factor 1-beta, KRAB-associated protein, TRIM28, KAP-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
20A1_PURE_TIF1b_Antibody_1_WB_072216
Whole cell lysates (15 µg protein) from HeLa, HEK293, and RAW264.7 cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-TIF1β (KAP-1, TRIM28) antibody, clone 20A1. Proteins were visualized using chemiluminescence detection by incubating with HRP goat anti-mouse IgG secondary antibody (1:3000 dilution, Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: MW ladder. Cell lysates were loaded in order of increasing TRIM28 mRNA expression levels; HEK293 cells express ~8-fold more TRIM28 mRNA than HeLa cells (source: Human Protein Atlas). Lane M: Molecular Weight marker.
  • 20A1_PURE_TIF1b_Antibody_1_WB_072216
    Whole cell lysates (15 µg protein) from HeLa, HEK293, and RAW264.7 cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-TIF1β (KAP-1, TRIM28) antibody, clone 20A1. Proteins were visualized using chemiluminescence detection by incubating with HRP goat anti-mouse IgG secondary antibody (1:3000 dilution, Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: MW ladder. Cell lysates were loaded in order of increasing TRIM28 mRNA expression levels; HEK293 cells express ~8-fold more TRIM28 mRNA than HeLa cells (source: Human Protein Atlas). Lane M: Molecular Weight marker.
  • 20A1_PURE_TIF1b_Antibody_2_IF_022514
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of purified anti-TIF1β (KAP-1, TRIM28) (clone 20A1) in blocking buffer overnight at 4°C, followed by DyLight™ 594 anti-mouse IgG (red) and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 20X objective.
Cat # Size Price Quantity Avail. Save
619301 25 µg $85
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619302 100 µg $185
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Description

TIFβ (transcription intermediary factor 1-beta) is an 88 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with SETDB1 and CBX3 proteins. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated
ChIP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.1 µg per ml. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence1, Western blotting1 and chromatin immunoprecipitation1.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

This clone may produce increased background by western blot when used at concentrations exceeding 0.1 μg/mL.

Application References

(PubMed link indicates BioLegend citation)
  1. Chang CW, et al. 2008 BMC Mol. Biol. 9:61. (WB, IF, ChIP)
RRID
AB_2209724 (BioLegend Cat. No. 619301)
AB_2209723 (BioLegend Cat. No. 619302)

Antigen Details

Structure
Tripartite motif family, contains three zinc-binding domains, a RING, a B-box type 1 and 2 domain, and a coiled-coil region. Predicted molecular weight approximately 110 kD
Distribution

Nuclear, associates with specific chromatin regions

Function
Forms a complex with KRAB-domain transcription factor and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Ryan RF, et al. 1999. Mol. Cell. Biol. 19:4366.
2. Schultz DC, et al. 2002. Genes Dev. 16:919.
3. Moosmann PR, et al. 1996. Nucleic Acids Res. 24:4859.
4. Friedman JR, et al. 1996. Genes Dev. 10:2067.
5. Messerschmidt DM, et al. 2012. Science 335:1499.
6. Chikuma S, et al. 2012. Nat. Immunol. 13:596.

 

Gene ID
10155 View all products for this Gene ID
UniProt
View information about TIF1β on UniProt.org

Related FAQs

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Go To Top Version: 8    Revision Date: 05/31/2018

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