Purified anti-TFIIB Antibody

Pricing & Availability
Clone
IIB8 (See other available formats)
Other Names
Transcription initiation factor IIB, General transcription factor TFIIB, S300-II, GTF2B, TF2B, TFIIB
Isotype
Mouse IgG2a
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Product Citations
publications
IIB8_Purified_TFIIB_Antibody_2_WB_050118
Total lysates (15µg protein) from HeLa (Lane 1), 20nM scrambled siRNA (lane 2), 5nM (Lane 3)and 20nM (Lane 4) TFIIB siRNA treated HeLa cells by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-TFIIB antibody, clone IIB8 (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder.
  • IIB8_Purified_TFIIB_Antibody_2_WB_050118
    Total lysates (15µg protein) from HeLa (Lane 1), 20nM scrambled siRNA (lane 2), 5nM (Lane 3)and 20nM (Lane 4) TFIIB siRNA treated HeLa cells by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-TFIIB antibody, clone IIB8 (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder.
  • IIB8_Purified_TFIIB_Antibody_WB_050118
    Total lysates (15µg protein) from MCF-7, HeLa, HepG2, Molt-4 and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-TFIIB antibody, clone IIB8 (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder.
Cat # Size Price Quantity Avail. Save
668405 100 µg $275
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Description

The Transcription Factor IIB (TFIIB) is a general factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Wide mammalian
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
This antibody should be aliquoted and stored at -20°C. Avoid freeze/thaw cycles.
Application

WB - Quality tested
FA, Gel Supershift, IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes Softag 3 epitope tagged proteins and is a human transcription factor. Clone IIB8 recognizes epitope 52-105 (TQDPSRVG). Nonspecific bands over 250kDa may be observed in some cell lysates. 

Additional reported applications (for the relevant formats) include: functional assay, gel supershift, and immunoprecipatation.

Application References

(PubMed link indicates BioLegend citation)
  1. Thompson NE and Burgess RR. 1994. Protein Expr. Purif. 5:468.
  2. Thompson NE, et al. 1995. J. Biol. Chem. 270:4735.
  3. Duellman SJ, et al. 2004. Protein Expr. Purif. 35:147.
RRID
AB_2566803 (BioLegend Cat. No. 668405)

Antigen Details

Structure
TFIIB is a 35 kD human transcription factor.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
2959 View all products for this Gene ID
UniProt
View information about TFIIB on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/01/2018

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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