Purified anti-TDP43 Antibody (Previously Covance catalog# SIG-39854)

Pricing & Availability
Clone
TDP2H4 (See other available formats)
Other Names
TAR DNA-binding protein-43, TDP 43, TARDBP, ALS10
Previously
Covance Catalog# SIG-39854
Isotype
Rat IgG2a
Ave. Rating
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Product Citations
publications
TDP2H4_PURE_TDP43_1_121417
IHC staining of anti-TDP43 antibody (clone TDP2H4) on formalin-fixed paraffin-embedded rat (A) and mouse (B) brain tissues. Following antigen retrieval using Sodium Citrate H.I.E.R and 0.25% Triton X-100, tissues were incubated with 2 µg/ml of the primary antibody overnight at 4°C. After incubation with biotinylated anti-rat antibody, the tissue was labeled with BioLegend’s HRP labeling reagent followed by addition of DAB chromogen (Cat. No. 926506) for detection and hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar 50 µm.
  • TDP2H4_PURE_TDP43_1_121417
    IHC staining of anti-TDP43 antibody (clone TDP2H4) on formalin-fixed paraffin-embedded rat (A) and mouse (B) brain tissues. Following antigen retrieval using Sodium Citrate H.I.E.R and 0.25% Triton X-100, tissues were incubated with 2 µg/ml of the primary antibody overnight at 4°C. After incubation with biotinylated anti-rat antibody, the tissue was labeled with BioLegend’s HRP labeling reagent followed by addition of DAB chromogen (Cat. No. 926506) for detection and hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar 50 µm.
  • TDP2H4_PURE_TDP43_2_121417
    Western blot of anti-TDP43 antibody (clone TDP2H4). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysates; Lane 4: 20 µg of rat brain lysates; Lane 5: 20 µg of Hela lysates. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by the incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405).
  • TDP2H4_PURE_TDP43_3_121417
    Western blot of anti-TDP43 antibody (clone TDP2H4). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum lysate; Lane 3: 20 µg of human brain nuclear fraction. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by the incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). The anti-α-tubulin antibody (Cat. No. 909601) was used as a loading control.
  • TDP2H4_PURE_TDP43_4_121417
    Direct ELISA of purified anti-TDP43 antibody (clone TDP2H4) binding to plate-immobilized full-length, N-terminal fragment (a.a. 1-207), or C-terminal fragment (a.a 208-414) of TDP43. ELISA was performed by coating wells with 100 ng of each protein. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-rat secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine) was used as the detection system.
Cat # Size Price Quantity Avail. Save
808301 100 µl $315
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Description

TDP43, also known as TARDBP, was originally identified as a protein that binds to the transactivation response (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome. TDP43 contains two copies of a ~90 amino acid RNA recognition motif (RRM) found in many proteins, which bind single stranded RNA. Recent research has shown that hyperphosphorylated, ubiquitinated, and N-terminally truncated TDP43 is the pathological hallmark lesion in most familial and sporadic forms of FTLD-U and ALS (1).

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This antibody was raised against a nonphosphorylated peptide corresponding to the C-terminal domain of human TDP43, which includes serine residues 409 and 410.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB, Direct ELISA - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution of 1:500 is suggested. For Western blotting, the suggested use of this reagent is 1.0 – 5.0 µg per ml. For Direct ELISA application, a concentration range of 10 ng - 1.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.​

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), and Direct ELISA.

Expected Staining Pattern: Nuclear

Application References

(PubMed link indicates BioLegend citation)
  1. Neumann M. 2009. Int. J. Mol. Sci. 10.3390/ijms10010232.
RRID
AB_2564740 (BioLegend Cat. No. 808301)

Antigen Details

Structure
TDP43 is a 414 amino acid protein with an apparent molecular mass of 45 kD.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
TDP43
Gene ID
23435 View all products for this Gene ID 230908 View all products for this Gene ID 100360075 View all products for this Gene ID 100174676 View all products for this Gene ID
UniProt
View information about TDP43 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 12/12/2017

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