Purified anti-Tau Nitrated (Tyr18) Antibody (Previously Covance catalog# SIG-39436)

Pricing & Availability
Clone
Tau-nY18 (See other available formats)
Other Names
Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle protein, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1
Previously
Covance Catalog# SIG-39436
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
Tau-nY18_Purified_Tau-Nitrated_Antibody_1_122718.png
IHC staining of purified anti-Tau Nitrated (Tyr18) antibody (clone Tau-nY18) on formalin-fixed paraffin-embedded Alzheimer's disease human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Tau-nY18_Purified_Tau-Nitrated_Antibody_1_122718.png
    IHC staining of purified anti-Tau Nitrated (Tyr18) antibody (clone Tau-nY18) on formalin-fixed paraffin-embedded Alzheimer's disease human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Tau-nY18_Purified_Tau-Nitrated_Antibody_2_122718.png
    IHC staining of purified anti-Tau Nitrated (Tyr18) antibody (clone Tau-nY18) on formalin-fixed paraffin-embedded Alzheimer's disease human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
829703 25 µl $95
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829701 100 µl $347
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Description

Tau proteins are microtubule-associated protein (MAPs) which are abundant in neurons of the central nervous system, but are also expressed at very low levels in CNS astrocytes and oligodendrocytes and elsewhere. One of tau's main functions is to modulate the stability of axonal microtubules. Tau is active primarily in the distal portions of axons providing microtubule stabilization as well as flexibility. Pathologies and dementias of the nervous system such as Alzheimer's disease feature tau proteins that have become defective and no longer stabilize microtubules properly. As a result, tau forms aggregates with specific structural properties referred to as Paired Helical Filaments (PHFs) that are a characteristic of many different types of dementias, known as tauopathies.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution; no preservatives or carrier proteins.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB, ELISA - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is specific for Tau nitrated at Tyr18. It does not cross react with wild type tau, or tau nitrated at Tyr 29, Tyr 197, or Tyr 394.

Application References

(PubMed link indicates BioLegend citation)
  1. Reyes J., et al. Selective Tau Tyrosine Nitration in Non-AD Tauopathies. Acta Neuropathol 123(1): 119-132, 2012.
  2. Reyes J., et al. A Possible Link Between Astrocyte Activation and Tau Nitration in Alzheimer's Disease. Neurobiol Dis 31(2): 198-208, 2008.
  3. Reynolds M., et al. Tau Nitration Occurs at Tyrosine 29 in the Fibrillar Lesions of Alzheimer's Disease and Other Tauopathies. J Neurosci 26(42): 10636-10645, 2006.
  4. Reyes JF, et al. 2008. Neurobiol. Dis. 31:198. (ELISA, WB IHC)
  5. Reyes JF, et al. 2012. Acta. Neuropathol. 123:119. (WB, IHC, ELISA)
RRID
AB_2721757 (BioLegend Cat. No. 829703)
AB_2564931 (BioLegend Cat. No. 829701)

Antigen Details

Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Stem Cells
Molecular Family
Tau
Gene ID
NA
UniProt
View information about Tau Nitrated (Tyr18) on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 08/11/2016

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