- 5C7 (See other available formats)
- Other Names
- Microtubule-associated protein tau, PHF-tau, Paired Helical Filament tau
Covance Catalog# MMS-5019
- Mouse IgG1, λ
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Tau proteins are microtubule-associated protein (MAPs) which are abundant in neurons of the central nervous system, but are also expressed at very low levels in CNS astrocytes and oligodendrocytes and elsewhere. One of tau's main functions is to modulate the stability of axonal microtubules. Tau is active primarily in the distal portions of axons providing microtubule stabilization as well as flexibility. Pathologies and dementias of the nervous system such as Alzheimer's disease, feature tau proteins that have become defective and no longer stabilize microtubules properly. As a result, tau forms aggregates with specific structural properties referred to as Paired Helical Filaments (PHFs) that are a characteristic of many different types of dementias, known as tauopathies.
Tau has two primary ways of controlling microtubule stability: isoforms and phosphorylation. Six tau isoforms exist in human brain tissue, and they are distinguished by the number of binding domains. Three isoforms have three binding domains and the remaining three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (for binding to the negatively-charged microtubule). Tau isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.
- Antibody Type
- Host Species
- This monoclonal antibody was raised against full-length human tau. The epitope was mapped to amino acids 267-278 of human 2N4R Tau (the longest isoform).
- Phosphate-buffered solution.
- The antibody was purified by affinity chromatography.
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
Tissue: Formalin-fixed Paraffin-embedded
Primary Incubation: 24 hours at 4°C
- Application Notes
This antibody is effective in immunoblotting (WB), and immunohistochemistry (IHC) on frozen and paraffin sections.
Clone 5C7 recognizes all 6 isoforms of Tau.
Positive Control: Human Alzheimer's Brain
AB_2715846 (BioLegend Cat. No. 814502)
AB_2564784 (BioLegend Cat. No. 814501)
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