Purified anti-Tau, 267-278 Antibody (Previously Covance catalog# MMS-5019)

Pricing & Availability
Clone
5C7 (See other available formats)
Other Names
Microtubule-associated protein tau, PHF-tau, Paired Helical Filament tau
Previously
Covance Catalog# MMS-5019
Isotype
Mouse IgG1, λ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
814502 25 µl $95
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814501 100 µl $234
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Description

Tau proteins are microtubule-associated protein (MAPs) which are abundant in neurons of the central nervous system, but are also expressed at very low levels in CNS astrocytes and oligodendrocytes and elsewhere. One of tau's main functions is to modulate the stability of axonal microtubules. Tau is active primarily in the distal portions of axons providing microtubule stabilization as well as flexibility. Pathologies and dementias of the nervous system such as Alzheimer's disease, feature tau proteins that have become defective and no longer stabilize microtubules properly. As a result, tau forms aggregates with specific structural properties referred to as Paired Helical Filaments (PHFs) that are a characteristic of many different types of dementias, known as tauopathies.



Tau has two primary ways of controlling microtubule stability: isoforms and phosphorylation. Six tau isoforms exist in human brain tissue, and they are distinguished by the number of binding domains. Three isoforms have three binding domains and the remaining three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (for binding to the negatively-charged microtubule). Tau isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against full-length human tau. The epitope was mapped to amino acids 267-278 of human 2N4R Tau (the longest isoform).
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IHC

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:40 
IHC: 1:50

Tissue: Formalin-fixed Paraffin-embedded 
Pretreatment: Citrate
Primary Incubation: 24 hours at 4°C

Application Notes

This antibody is effective in immunoblotting (WB), and immunohistochemistry (IHC) on frozen and paraffin sections.

Clone 5C7 recognizes all 6 isoforms of Tau.

Positive Control: Human Alzheimer's Brain

RRID
AB_2715846 (BioLegend Cat. No. 814502)
AB_2564784 (BioLegend Cat. No. 814501)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 05/05/2016

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