Purified anti-TAL1 Antibody

Pricing & Availability
Clone
4G8C07 (See other available formats)
Other Names
T-cell acute lymphocytic leukemia 1, Class A basic helix-loop-helix protein 17 (bHLHa17), T-cell leukemia/lymphoma protein 5 (TCL5), SCL
Isotype
Mouse IgG1, κ
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Product Citations
publications
4G8C07_PURE_TAL1_Antibody_1_WB_093015
Total cell lysate from human Jurkat cells (lane 1), 293T cells (lane 2), and mouse thymus (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-TAL1 (clone 4G8C07) antibody. Proteins were visualized using an HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 4G8C07_PURE_TAL1_Antibody_1_WB_093015
    Total cell lysate from human Jurkat cells (lane 1), 293T cells (lane 2), and mouse thymus (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-TAL1 (clone 4G8C07) antibody. Proteins were visualized using an HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 4G8C07_PURE_TAL1_Antibody_2_ICC_042419
    Jurkat cells were stained with purified anti-TAL1 (clone 4G8C07) antibody, followed by staining with Alexa Fluor® 594 conjugated goat anti-mouse IgG (red) antibody. Nuclei were stained with DAPI (blue).
Cat # Size Price Quantity Avail. Save
681001 25 µg $95
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681002 100 µg $245
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Description

TAL1, also known as SCL, is a class I basic helix-loop-helix (bHLH) transcription factor. TAL1 is essential for the establishment of hematopoietic stem cells during embryogenesis, and regulating proliferation and differentiation of multiple hematopoeitic cell lineages, such as erythrocytes, megakaryocytes, mast cells, and eosinoohils. Tal1-null mice are embryonic lethal due to the failure of yolk sac hematopoiesis and vascular defects. In hematopoietic cells, TAL1 forms a multiprotein complex with other transcription factors, which include the E protein TCF3, LMO2, LDB1, and GATA family members. The large protein complex containing TAL1 binds to specific cis-regulatory elements encoding the consensus sequence AACAGATGGT and regulates expression of target genes. TAL1 is not expressed in normal lymphoid cells, but ectopic expression of TAL1 in T-cells leads to T-cell acute lymphocytic leukemia (T-ALL).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human TAL1 recombinant protein (1-263 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566441 (BioLegend Cat. No. 681001)
AB_2566442 (BioLegend Cat. No. 681002)

Antigen Details

Structure
331 amino acids with a predicted molecular weight of 34.3 kD. Contains a helix-loop-helix (bHLH) domain that is responsible for DNA binding and protein to protein interaction.
Distribution

Nucleus.

Function
TAL1 is a bHLH transcription factor that regulates hematopoietic and vascular development. Ectopic expression of TAL1 is linked to T-cell acute lymhoblastic leukemia (T-ALL).
Interaction
Forms heterodimers with TCF3. Interacts with ETS1, LMO2, and DRG1.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Wu W, et al. 2014. Genome Res. 24:1945.
2. Kolodziej S, et al. 2014. Nat. Commun. 5:3995.
3. Palii CG, et al. 2014. Cell Stem Cell 14:644.
4. Yun WJ, et al. 2014. Nucleic Acids Res. 42:4283.
5. Zhou Y, et al. 2013. Blood 26:4199.
6. Sanda T, et al. 2012. Cancer Cell 22:209.

Gene ID
6886 View all products for this Gene ID
UniProt
View information about TAL1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/24/2019

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