Purified anti-Synaptotagmin-12 Antibody (Previously Covance catalog# MMS-5197)

Pricing & Availability
Clone
N277/7 (See other available formats)
Other Names
Synaptotagmin-12, sytXII, synaptotagmin 12, Syt12, Synaptotagmin XII, Synaptotagmin-related gene 1 protein, Srg1, Sytr1
Previously
Covance Catalog# MMS-5197
Isotype
Mouse IgG1
Ave. Rating
0 reviews
N2777_PURE_Synaptotagmin12_Antibody_HR_1_090517.jpg
Western blot of anti-Synaptotagmin-12 antibody (clone N277/7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with clone N277/7 at 10 µg/ml, overnight at 4°C, followed by incubation with a HRP conjugated secondary antibody. Enhanced Chemiluminescence was used for detection.
  • N2777_PURE_Synaptotagmin12_Antibody_HR_1_090517.jpg
    Western blot of anti-Synaptotagmin-12 antibody (clone N277/7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with clone N277/7 at 10 µg/ml, overnight at 4°C, followed by incubation with a HRP conjugated secondary antibody. Enhanced Chemiluminescence was used for detection.
  • N2777_PURE_Synaptotagmin12_Antibody_HR_2_090517.jpg
    IHC staining of anti-Synaptotagmin-12 antibody (clone N277/7) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate Heat Induced Epitope Retrieval (H.I.E.R.), the tissue was incubated with clone N277/7 at 10 µg/ml overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) was used for detection following by hematoxylin and bluing solution counterstain.
  • N2777_PURE_Synaptotagmin12_Antibody_HR_3_090517.jpg
    IHC staining of anti-Synaptotagmin-12 antibody (clone N277/7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate Heat Induced Epitope Retrieval (H.I.E.R.), the tissue was incubated with clone N277/7 at 10 µg/ml overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) was used for detection following by hematoxylin and bluing solution counterstain.
Cat # Size Price Quantity Avail. Save
820401 100 µl $140.00
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Description

Synaptotagmin 12 (Syt12) is regulated by protein kinase A (PKA) and is unique from other synaptotagmins in that it does not bind to Ca2+. Syt12 interaction with Syt1 prevents SNARE complex binding. Expression of Syt12 induces spontaneous neurotransmitter release, providing a mechanism for spontaneous synaptic-vesicle exocytosis that is Ca2+-independent.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat, Human
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 168-255 (cytoplasmic C2A domain) of mouse Synaptotagmin-12.
Formulation
Phosphate-buffered solution with BSA + 0.1% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IHC

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 2 - 10 µg/ml
IHC: 10 µg/ml

Tissue: Formalin-fixed Paraffin-embedded
Pre-treatment: Citrate
Primary incubation: 24 hours at 2-8°C

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

*Expected MW: 47 kD

WB Positive Control: Brain membrane lysates from human, mouse and rat

IHC Positive Control: Rat and mouse brain  

RRID
AB_2564840 (BioLegend Cat. No. 820401)

Related FAQs

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Go To Top Version: 2    Revision Date: 07/14/2016

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