Purified anti-Synapsin I/II/III Antibody

Pricing & Availability
Clone
A17080A (See other available formats)
Other Names
SYN, Synapsin, Synapsin I, Synapsin II, Synapsin III
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1_A17080A_PURE_Synapsin_Antibody_WB_072018
Western blot of purified anti-Synapsin I/II/III antibody (clone A17080A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 0.005 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_A17080A_PURE_Synapsin_Antibody_WB_072018
    Western blot of purified anti-Synapsin I/II/III antibody (clone A17080A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 0.005 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17080A _PURE_Synapsin_Antibody_IHC-P_1_072018
    IHC staining of purified anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A17080A_PURE_Synapsin_Antibody_IHC-P_2_072018
    IHC staining of purified anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
853701 25 µg $105
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Description

Synapsins are a family of proteins involved in neurotransmitter release at the chemical synapses. Synapsins anchor synaptic vesicles to the cytoskeletal structures at the presynaptic terminal. Upon receiving an action potential, phosphorylation of synapsins, possibly by PKA, leads to the release of synaptic vesicles from cytoskeletal structures. This allows membrane docking and fusion of synaptic vesicles with the presynaptic membrane and neurotransmitter release. 

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein corresponding to amino acids 362-450 of human Synapsin I
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.001 - 0.005 µg per ml. For immunohistochemistry, a concentration range of 0.1 - 0.5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2749954 (BioLegend Cat. No. 853701)

Antigen Details

Structure
Synapsin I is a 704 amino acid protein with an apparent molecular mass of ~74 kD. Synapsin II is a 582 amino acid protein with an apparent molecular mass of ~63 kD. Synapsin III is a 580 amino acid protein with an apparent molecular mass of ~63 kD.
Distribution

Tissue distribution: Mainly in the central nervous system. Also expressed in testis.
Cellular distribution: Cytosol, nucleus, and plasma membrane.

Function
Synapsins are involved in the regulation of synaptic vesicle fusion and neurotransmitter release at the presynaptic terminals.
Interaction
CAPON (Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein), PKA
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References
  1. Greengard P, et al. 1993. Science. 259(5096):780
  2. Milovanovic D, et al. 2017. Neuron. 93(5):995
  3. Evergren E, et al. 2007. J. Neurosci. Res. 85(12):2648
Gene ID
6853 View all products for this Gene ID 6854 View all products for this Gene ID 8224 View all products for this Gene ID
UniProt
View information about Synapsin I/II/III on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07/20/2018

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