Purified anti-SVOP Antibody (Previously Covance catalog# MMS-5195)

Pricing & Availability
Clone
N356/9 (See other available formats)
Other Names
Synaptic vesicle 2-related protein, SV2-related protein
Previously
Covance Catalog# MMS-5195
Isotype
Mouse IgG2b
Ave. Rating
0 reviews
Product Citations
publications
N3569_pure_SVOP_Antibody_1_020918
Western blot of purified anti-SVOP antibody (clone N356/9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 5 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N3569_pure_SVOP_Antibody_1_020918
    Western blot of purified anti-SVOP antibody (clone N356/9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 5 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N3569_pure_SVOP_Antibody_2_020918
    IHC staining of purified anti-SVOP antibody (clone N356/9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • N3569_pure_SVOP_Antibody_3_020918
    IHC staining of purified anti-SVOP antibody (clone N356/9) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N3569_pure_SVOP_Antibody_4_020918
    IHC staining of purified anti-SVOP antibody (clone N356/9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
819902 25 µl $70.00
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819901 100 µl $140.00
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Description

Synaptic vesicle (SV) 2 -related protein (SVOP), is protein mainly expressed in the brain and endocrine cells and may be involved in neurotransmitter transport in SV. SVOP is expressed early in development and is the protein that decreases in expression levels the most during aging.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat, Human
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-85 of the cytoplasmic N-terminus of rat SVOP.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and paraffin-embedded immunohistochemical staining (IHC-P).

RRID
AB_2734582 (BioLegend Cat. No. 819902)
AB_2564835 (BioLegend Cat. No. 819901)

Antigen Details

Structure
Expected MW: 60 kD
Gene ID
NA
UniProt
View information about SVOP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02/09/2018

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