- BL26882 (See other available formats)
- Regulatory Status
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.Product Details
- Strep-Tag fusion proteins
- Antibody Type
- Host Species
- Synthetic Peptide
- PBS pH: 7.4, containing 0.5 % BSA, 0.02% sodium azide, and 50% glycerol.
- The antibody was affinity purified from mouse ascites by affinity chromatography using specific immunogen.
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20Â°C.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
AB_2629594 (BioLegend Cat. No. 688202)
- The amino acid sequence of the Strep-tag is: Trp-Ser-His-Pro-Gln-Phe-Glu-Lys.
- Strep-tag can be attached to the N- or C-terminus of protein to become Strep-tag fused protein.
- Biology Area
- Cell Biology
- Antigen References
1. Skerra A, et al. 2000. Methods Enzymol. 326:271.
2. Schmidt TG, et al. 2007. Nat. Protoc. 2:1528.
- Gene ID
- View information about Strep-Tag on UniProt.org
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