Purified anti-Stonin-2 Antibody (Previously Covance catalog# MMS-5270)

Pricing & Availability
Clone
N346/9 (See other available formats)
Other Names
Stonin-2, stoned B homolog 2, homolog of stoned B, STON2, STN2 and STNB
Previously
Covance Catalog# MMS-5270
Isotype
Mouse IgG2b
Ave. Rating
0 reviews
N3469_Pure_Stronin-2_Antibody_1_122017
Western blot of anti-Stonin-2 antibody (clone N346/9) and mouse IgG2b isotype control. Lane 1, 5: Molecular weight marker; Lane 2, 6: 20 µg of human brain membrane lysate; Lane 3, 7: 20 µg of mouse brain membrane lysate; Lane 4, 8: 20 µg of rat brain membrane lysate. The blots were incubated with 0.05 µg/mL of clone N346/9 or mouse IgG2b overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N3469_Pure_Stronin-2_Antibody_1_122017
    Western blot of anti-Stonin-2 antibody (clone N346/9) and mouse IgG2b isotype control. Lane 1, 5: Molecular weight marker; Lane 2, 6: 20 µg of human brain membrane lysate; Lane 3, 7: 20 µg of mouse brain membrane lysate; Lane 4, 8: 20 µg of rat brain membrane lysate. The blots were incubated with 0.05 µg/mL of clone N346/9 or mouse IgG2b overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N3469_Pure_Stronin-2_Antibody_2_122017
    Human kidney IHC staining of anti-Stonin-2 antibody (clone N346/9) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s ACUITYAdvanced Biotin Free Polymer Detection System DAB Kit (Cat. No. 931001) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • N3469_Pure_Stronin-2_Antibody_3_122017
    Mouse Tissue IHC staining of anti-Stonin-2 antibody (clone N346/9) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s ACUITYAdvanced Biotin Free Polymer Detection System DAB Kit (Cat. No. 931001) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • N3469_Pure_Stronin-2_Antibody_4_122017
    Rat Tissue IHC staining of anti-Stonin-2 antibody (clone N346/9) on formalin-fixed paraffin-embedded rat kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s ACUITYAdvanced Biotin Free Polymer Detection System DAB Kit (Cat. No. 931001) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • N3469_Pure_Stronin-2_Antibody_5_122017
    Staining of Stonin2 MMS-5270 (Green) on methanol fixed human SY5Y cells at 10 µg/ml
Cat # Size Price Quantity Avail. Save
804701 100 µl $140.00
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Description

Stonin-2 (STON2), also known as stoned B homolog 2 (STN2 and STNB), is an adaptor-like protein involved in endocytosis. Stonin-2 is regulated by AP-2 and facilitates internalization of synaptotagmin for recycling within the neuron.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-555 (N-terminus) of human Stonin-2.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P, ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.05 µg per ml. For immunohistochemistry, a concentration of 10 µg/ml is suggested. For immunocytochemistry, a dilution range of 1:100 - 1:500 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), and immunocytochemistry (ICC).

RRID
AB_2564671 (BioLegend Cat. No. 804701)

Antigen Details

Structure
Stonin-2 has an apparent molecular mass of 120 kD.
Distribution

Cellular distribution: Endocytic, synaptic and cytoplasmic membranes, cytosol and nucleus.

Gene ID
85439 View all products for this Gene ID
UniProt
View information about Stonin-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 12/20/2017

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