Purified anti-STAT5 Phospho (Tyr694) Antibody

Pricing & Availability
Clone
Poly6511 (See other available formats)
Other Names
Signal transducer and activator of transcription 5 (STAT5), MGF
Isotype
Rabbit Polyclonal IgG
Ave. Rating
Submit a Review
Product Citations
publications
Poly6511_STAT5-pY694_090611
U937 whole cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with polyclonal anti-STAT5 pY694 (Poly6511) antibody. Proteins were visualized using anti-rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection. Lane 1 - lysate from untreated U937 cells treated with 10 ng/ml human GM-CSF for 30 minutes. The same blot was stripped and re-probed with β-actin antibody (Poly6221) to ensure equal amounts of lysates were loaded in both lanes.
  • Poly6511_STAT5-pY694_090611
    U937 whole cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with polyclonal anti-STAT5 pY694 (Poly6511) antibody. Proteins were visualized using anti-rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection. Lane 1 - lysate from untreated U937 cells treated with 10 ng/ml human GM-CSF for 30 minutes. The same blot was stripped and re-probed with β-actin antibody (Poly6221) to ensure equal amounts of lysates were loaded in both lanes.
Cat # Size Price Quantity Avail. Save
651101 25 µL $105
Check Availability


Need larger quantities of this item?
Request Bulk Quote
651102 100 µL $225
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

STAT5 has gene transactivation activity and plays a key role in lymphocyte differentiation and proliferation. Tyrosine phosphorylation of STAT5, induced by cytokine stimulation, results in dimerization through its SH2 domains. The dimerized STAT5 proteins translocates from the cytoplasm to the nucleus and subsequently regulates gene transcription. 

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Modified synthetic peptide
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store undiluted at -20°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The STAT5 phospho-tyr694 antibody recognizes the regulatory tyrosine phosphorylation of human STAT5 protein and has been shown to be useful for Western blotting.


This product may contain other non-IgG subtypes.

RRID
AB_10896060 (BioLegend Cat. No. 651101)
AB_10900416 (BioLegend Cat. No. 651102)

Antigen Details

Structure
STAT5 exists in two isoforms, STAT5A and STAT5B, which consists of an N-terminal dimerization domain, a SH2 domain, a regulatory tyrosine (Y694 and Y699 in the case of STAT5A and STAT5B, respectively) responsible for binding of SH2 domain, and a C-termina
Distribution

Cytoplasm. Translocates to nucleus in response to tyrosine phosphorylation.

Function
STAT5 is a transcription regulator and is involved in cell differentiation, proliferation, and survival, in response to several cytokines, such as interleukins, erythropoietin, growth hormone, thrombopoietin, and prolactin.
Interaction
Histone acetyltransferases CBP and p300; SMRT.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Phospho-Proteins, Nuclear Markers
Antigen References

1. Teglund S, et al. 1998. Cell 93:841.
2. Moriggl R, et al. 1999. Immunity 10:249.
3. Peschon JJ, et al. 1994. J. Exp. Med. 180:1955.
4. Lynn M, et al. 2011. Ann. N.Y. Acad. Sci. 1217:18.
5. Malin S, et al. 2010. Curr. Opin. Immunol. 22:168.

Gene ID
6777 View all products for this Gene ID
UniProt
View information about STAT5 Phospho Tyr694 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Forgot your password? Reset Password
Request an Account