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Purified anti-STAT2 Antibody

Pricing & Availability
Clone
W15088A (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 2, interferon alpha induced transcriptional activator
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_W15088A_PURE_STAT2_Antibody_110816
Total cell lysates (15 µg protein) from Jurkat, 293T and NIH3T3 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-STAT2 antibody (clone W15088A). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 1_W15088A_PURE_STAT2_Antibody_110816
    Total cell lysates (15 µg protein) from Jurkat, 293T and NIH3T3 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-STAT2 antibody (clone W15088A). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 2_W15088A_GoChIP_STAT2_Antibody_1_052118
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 U266 cells treated with IFNα2 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT2 Antibody (clone W15088A, Cat. No. 693303) or B) equal amount of Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MX1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 3_W15088A_GoChIP_STAT2_Antibody_2_052118.
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 U266 cells treated with IFNα2 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT2 Antibody (clone W15088A, Cat. No. 693303) or B) equal amount of Purified Mouse IgG2b, κ isotype control antibody (Clone MPC-11, Cat. No. 400301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IFNB1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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693302 100 µg $253
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Description

STAT2, a 113 kD member of the STAT (signal transducer and activators of transcription) protein family, is phosphorylated in response to interferon alpha receptor signaling by Janus kinase I. Phosphorylation of STAT2 induces nuclear translocation to activate transcription. STAT2 is highly expressed in the skin and thymus. STAT 2 forms complex with STAT1 and p48 to activate transcription and does not bind DNA directly. STAT3 interacts with interferon regulatory factor 9, interferon alpha receptor, STAT1, p48, and CREB binding protein.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant protein of human STAT2 (671-851 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ChIP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For ChIP applications, the suggested dilution is 1:50 - 1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W15088A does not cross-react with mouse (in-house tested).

RRID
AB_2632834 (BioLegend Cat. No. 693302)

Antigen Details

Structure
STAT family, contains coiled coil and SH2 domain, 113 kD.
Distribution

Translocates into nucleus after phosphorylation. Expressed in skin, thymus.

Function
Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to nucleus to act as transcriptional activator. Interferon alpha binding to its receptor induces a transcription complex (ISGF3) composed of p48, STAT2, and STAT.
Interaction
STAT1, p48, interferon regulatory factor 9, interferon receptor 2, CREB binding protein.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Bluyssen HA, Levy DE. 1997. J. Biol. Chem. 272:4600.
2. Qureshi SA, et al. 1996. Mol. Cell. Biol. 16:288.

Gene ID
6773 View all products for this Gene ID
UniProt
View information about STAT2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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