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- Clone
- Sp3G11/1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Spastic paraplegia 4 protein, SPG4, ADPSP, FSP2
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-Spastin antibody (clone Sp3G11/1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 868201 | 25 µg | $106 | ||||
| 868202 | 100 µg | $276 | ||||
Spastin is a microtubule-severing protein that cleaves longer microtubules (MTs) to shorter ones. The severing of MT regulates its numbers and mobility, and the distribution of the plus-end. This has also been related to membrane trafficking of microtubules. Mutations in the spastin gene SPG4 is the main cause for hereditary spastic paraplegia, a neurodegenerative disorder mainly occurring in corticospinal tracks. Due to the presence of 2 start codons in SPG4 gene, 2 isoforms of spastin exist: a 68 kD isoform termed M1 and a 60 kD isoform termed M87. The M1 isoform is only detected in the adult spinal cord while the M87 isoform is ubiquitously expressed.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human spastin protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2814618 (BioLegend Cat. No. 868201)
AB_2814619 (BioLegend Cat. No. 868202)
Antigen Details
- Structure
- Human spastin is a 616 amino acid protein with a predicted molecular mass of 67 kD.
- Distribution
-
Tissue distribution: Expressed in brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle. The short isoform may predominate in the brain and spinal cord.
Cellular distribution: Cytoskeleton, endoplasmic reticulum, nucleus, and membrane. - Function
- Spastin is involved in the regulation of microtubule severing.
- Interaction
- Spastin mostly forms homohexamer.
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroscience
- Molecular Family
- Cytoskeletal Proteins
- Antigen References
-
- Hazan J. et al. 1999. Nat Genet. 23(3):296-303.
- Solowska JM. et al. 2014. J Neurosci. 34(5):1856-67.
- Gene ID
- 6683 View all products for this Gene ID
- UniProt
- View information about Spastin on UniProt.org
Related FAQs
Other Formats
View All Spastin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Spastin | Sp3G11/1 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Spastin
Western blot of purified anti-Spastin antibody (clone Sp3G11...
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