Purified anti-SOX-10 Antibody

Pricing & Availability
Clone
BSB-62 (See other available formats)
Other Names
Transcription factor SOX-10
Isotype
Mouse IgG2b, κ
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Product Citations
publications
BSBdash62_PURE_SOX10_Antibody_IHCP_1_080516
IHC staining of purified anti-SOX-10 antibody (clone BSB-62) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.
  • BSBdash62_PURE_SOX10_Antibody_IHCP_1_080516
    IHC staining of purified anti-SOX-10 antibody (clone BSB-62) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.
  • BSBdash62_PURE_SOX10_Antibody_IHCP_2_080516
    IHC staining of purified anti-SOX-10 antibody (clone BSB-62) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.
  • BSBdash62_PURE_SOX10_Antibody_IHCP_3_080516
    IHC staining of purified anti-SOX-10 antibody (clone BSB-62) on formalin-fixed paraffin-embedded rat and mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody for overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • BSBdash62_PURE_SOX10_Antibody_IHCP_4_080516
    IHC staining of anti-SOX-10 antibody (clone BSB-62) on formalin-fixed paraffin-embedded human glial tumor tissue. Following antigen retrieval using sodium citrate, the tissue was incubated with the primary antibody for 30 minutes at room temperature at 10 µg/ml. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection, followed by counterstaining with hematoxylin.
Cat # Size Price Quantity Avail. Save
847201 25 µg $105
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847202 100 µg $265
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Description

SOX-10 is a transcriptional activator that is involved in the regulation of embryonic development and in the determination of cell fate.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to the N-terminus of human SOX-10.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody may show weaker reactivity to human tissues.

Product Citations
  1. Dravis C 2018. Cancer cell. 34:466. PubMed
Publication Library
RRID
AB_2734617 (BioLegend Cat. No. 847201)
AB_2629666 (BioLegend Cat. No. 847202)

Antigen Details

Structure
SOX-10 is a 466 amino acid protein with a molecular mass of 50 kD.
Distribution

Tissue distribution: primarily expressed in the nuclei of glia in fetal and adult brain, and in the heart, salivary gland and skin.
Cellular distribution: nucleus.

Function
SOX-10 likely functions synergistically with the POU domain protein TST-1/OCT6/SCIP to confer function of other transcription factors in developing and mature glia. The protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development.
Interaction
DNA, PAX3, NMI.
Cell Type
Microglia
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Endosomal Markers
Antigen References

1. Bondurand N, Sham MH. 2013. Dev. Biol. 382(1):330. PubMed
2. Mollaaghababa R, Pavan WJ. 2003. Oncogene. 22(20):3024. PubMed

Gene ID
6663 View all products for this Gene ID
UniProt
View information about SOX-10 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 03/23/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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