Purified anti-SOD1 Antibody

Pricing & Availability
Clone
O98B10 (See other available formats)
Other Names
Superoxide dismutase 1, Superoxide dismutase [Cu-Zn] , Superoxide Dismutase, Cystolic
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
O98B10_PURE_SOD1_Antibody_WB_083117
Western blots of anti-SOD1 antibody (clone O98B10) and isotype-matched control mouse IgG2b. Lanes 1 and 2: 20 µg of normal human brain lysates; Lanes 3 and 4: 20 µg of mouse brain lysates. Blots were incubated overnight at 4°C with 1 µg/ml of primary antibodies, followed by incubation with horseradish peroxidase labeled goat anti-mouse IgG secondary antibody. Enhanced Chemiluminescence was used as the detection system.
  • O98B10_PURE_SOD1_Antibody_WB_083117
    Western blots of anti-SOD1 antibody (clone O98B10) and isotype-matched control mouse IgG2b. Lanes 1 and 2: 20 µg of normal human brain lysates; Lanes 3 and 4: 20 µg of mouse brain lysates. Blots were incubated overnight at 4°C with 1 µg/ml of primary antibodies, followed by incubation with horseradish peroxidase labeled goat anti-mouse IgG secondary antibody. Enhanced Chemiluminescence was used as the detection system.
  • O98B10_PURE_SOD1_Antibody_WB_2_051519
    Western blot of purified anti-SOD1 antibody (clone O98B10). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 436302). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
850701 25 µg $90
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850702 100 µg $225
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Description

Superoxide dismutase 1 (SOD1) is one of three isozymes responsible for destroying free superoxide radicals in the body. SOD1 is a homodimeric cytoplasmic Cu/Zn binding protein that converts harmful superoxide radicals to oxygen and hydrogen peroxide. The other isozymes are SOD2 and SOD3, which are localized to the mitochondria and extracellular space, respectively. SOD1 mutations are associated with familial amyotrophic lateral sclerosis.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human SOD1 expressed in 293T cells transfected with human SOD1 expression vector.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only validated with the purified format.

RRID
AB_2716015 (BioLegend Cat. No. 850701)
AB_2716016 (BioLegend Cat. No. 850702)

Antigen Details

Structure
SOD1 is a homodimeric β-barrel protein that contains an intramolecular disulfide bond and a binuclear Cu/Zn site in each subunit. SOD1 monomers have an apparent molecular weight of ~16 kD.
Distribution

Tissue sources: Ubiquitously expressed.  
Cellular distribution: Cytosol, mitochondrion, peroxisome and nucleus.

Function
SOD1 converts harmful superoxide radicals in the body to oxygen and hydrogen peroxide.
Interaction
UBC, CCS, PRDX1, HSPA4
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Enzymes and Regulators
Antigen References

1. Lee S, Kim HJ. 2015. Exp. Neurobiol. 24(1):1-7.
2. Tafuri F, et al. 2015. Front Cell Neurosci. 9: 336.
3. Palomo, GM, Manfredi G. 2015. Brain Res. 1607:36-46.
4. Rosen DR, et al. 1993. Nature 362 (6415):59-62.

Gene ID
6647 View all products for this Gene ID
UniProt
View information about SOD1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 05/15/2019

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