- O91D3/SNAP-25 (See other available formats)
- Other Names
- SNAP-25, Synaptosomal-associated protein 25
- Mouse IgG2a, κ
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Synaptosomal-associated protein 25 (SNAP-25) is a component of the trans-SNARE (t-SNARE) complex. SNAP-25 has been reported to account for the specificity of membrane fusion, and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP-25 has been shown to inhibits both ATP-dependent and independent Ca2+-triggered release of glutamate from central nervous system (CNS) synaptosomal membranes. This indicates that SNAP-25 has a role not only in the formation of the synaptic vesicle-target v- and t-SNARE complex, but also in the final neurotransmitter release.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Full-length recombinant human SNAP-25 protein (NP_003072) produced in HEK293T cell.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IHC-P - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
AB_2715872 (BioLegend Cat. No. 850301)
AB_2715873 (BioLegend Cat. No. 850302)
- SNAP-25 is a 206 amino acid protein with a molecular mass of 23 kD.
Tissue distribution: Mainly in the central nervous system, in neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei and granule cells of the cerebellum.
Cellular distribution: Cytosol, golgi apparatus and cell membrane.
- SNAP-25 associates with proteins involved in vesicle docking and membrane fusion. It regulates plasma membrane recycling through its interaction with centromere protein F (CENPF).
- SNAP-25 has been found to be part of the SNARE core complex containing SNAP25, VAMP2 and STX1A). SNAP-25 was also found in a complex containing SYT1, SV2B and syntaxin-1. SNAP-25 interacts with KCNB1 (via N-terminus), which reduces the voltage-dependent potassium channel KCNB1 activity in pancreatic beta cells. In developing brain and cell cultures, SNAP-25 reacts with neuronal cell bodies and axons. It is useful as an early marker for developing neurons in cell culture.
- Cell Type
- Mesenchymal Stem Cells, Neurons
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Presynaptic proteins
- Antigen References
1. Egbujo CN, et al. 2016. Curr. Psychiatry Rep. 18(8):77.
2. Antonucci F, et al. 2016. Front. Synaptic Neurosci. 8:7.
3. Cupertino RB, et al. 2016. J. Neural. Transm. (Vienna). 123(8):867.
- Gene ID
- 6616 View all products for this Gene ID
- View information about SNAP-25 on UniProt.org
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