Purified anti-SNAP-25 Antibody (Previously Covance catalog# MMS-614P)

Pricing & Availability
Clone
SP12 (See other available formats)
Other Names
Synaptosomal-associated protein 25, SUP, RIC-4, RIC4, SEC9, SNAP
Previously
Covance Catalog# MMS-614P
Isotype
Mouse IgG1, κ
Ave. Rating
2 reviews
Product Citations
publications
A_SP12_PURE_SNAP25_IHCP_HUCerebellum_113018
IHC staining of purified anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A_SP12_PURE_SNAP25_IHCP_HUCerebellum_113018
    IHC staining of purified anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B_SP12_PURE_SNAP25_IHCP_HUPancreas_113018
    IHC staining of purified anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded human pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • C_SP12_PURE_SNAP25_IHCP_MUPancreas_113018
    IHC staining of purified anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded mouse pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • D_SP12_PURE_SNAP25_IHCP_RatBrain_113018
    IHC staining of purified anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • E_SP12_PURE_SNAP25_WB_113018
    Western blot of purified anti-SNAP-25 antibody (clone SP12). Lane 1: Molecular weight marker; Lane 2: 20 µg of SH-SY5Y cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
805002 25 µl $95
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805001 100 µl $234
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Description

SNAP-25, also known as synaptosomal-associated protein 25, is a component of the trans-SNARE complex. It is a Q-SNARE protein anchored to the membrane on the cytosolic face, and does not contain a trans-membrane domain. The SNARE complex is involved in the vesicle fusion process. SNAP-25 is implicated in the inhibition of voltage-gated calcium channels, and has been shown to decrease calcium responsiveness in glutamatergic synapses.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a crude synaptic preparation from the post mortem human brain.
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Validated
IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).

Application References

(PubMed link indicates BioLegend citation)
  1. Honer WG, et al. 1993. Brain Res. 609:9-20.
  2. Brinkmalm A, et al. 2014. Mol. Neurodegener. 9:53. (IP) PubMed
Product Citations
  1. Brinkmalm A, et al. 2014. Mol Neurodegener. 9:53. PubMed
Publication Library
RRID
AB_2728531 (BioLegend Cat. No. 805002)
AB_2564674 (BioLegend Cat. No. 805001)

Antigen Details

Structure
Expected MW: 28 kD
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
6616 View all products for this Gene ID
UniProt
View information about SNAP-25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 11/30/2018

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