Purified anti-SAP102 Antibody (Previously Covance catalog# MMS-5186)

Pricing & Availability
Clone
N19/2 (See other available formats)
Other Names
Disks large homolog 3, SAP102, SAP-102, discs large homolog 3, PSD-95/SAP90-related protein 1, synapse-associated protein 102, Dlgh3
Previously
Covance Catalog# MMS-5186
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
N192_purified_SAP102_Antibody_1_020518
Western blot of purified anti-SAP102 antibody (clone N19/2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N192_purified_SAP102_Antibody_1_020518
    Western blot of purified anti-SAP102 antibody (clone N19/2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N192_purified_SAP102_Antibody_2_020518
    IHC staining of purified anti-SAP102 antibody (clone N19/2) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N192_purified_SAP102_Antibody_3_020518
    IHC staining of purified anti-SAP102 antibody (clone N19/2) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N192_purified_SAP102_Antibody_4_020518
    IHC staining of purified anti-SAP102 antibody (clone N19/2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
832002 25 µl $70.00
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832001 100 µl $140.00
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Description

SAP-102, also known as DLG3 or neuroendocrine-DLG, is a member of the MAGUK (membrane-associated guanylate kinase) superfamily. It interacts with a number of adhesion molecules as well as glutamate receptors on the postsynaptic membrane, mediating scaffolding of synaptic signaling.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-120 of rat SAP102.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10.0 µg per ml. For immunohistochemistry, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).

RRID
AB_2564952 (BioLegend Cat. No. 832001)

Related FAQs

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Go To Top Version: 2    Revision Date: 06/23/2016

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