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Purified anti-S100B Antibody

Pricing & Availability
Clone
11C12E12 (See other available formats)
Regulatory Status
RUO
Other Names
S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
11C12E12_PURE_S100B_Antibody_WB_032416
Western blot analysis of rat brain (lane 1) and mouse brain (lane 2). Anti- S100B antibody (clone 11C12E12 ) was used, followed by HRP Goat anti-mouse IgG (minimal x-reactivity) antibody. Purified anti-GAPDH antibody was used as a loading control. We are not sure about the specificity of the high molecular weight band.
  • 11C12E12_PURE_S100B_Antibody_WB_032416
    Western blot analysis of rat brain (lane 1) and mouse brain (lane 2). Anti- S100B antibody (clone 11C12E12 ) was used, followed by HRP Goat anti-mouse IgG (minimal x-reactivity) antibody. Purified anti-GAPDH antibody was used as a loading control. We are not sure about the specificity of the high molecular weight band.
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676603 25 µg $101
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676604 100 µg $253
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Description

S100B is glial-specific. It has been shown that it is only expressed by a subtype of mature astrocytes that ensheath blood vessels, and by NG2-expressing cells. This protein may function in neurite extension, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing central nervous system (CNS), it acts as a neurotrophic factor and neuronal survival protein. In the adult organism, it is usually elevated due to nervous system damage, which makes it a potential clinical marker. S100B is secreted by astrocytes or can spill from injured cells and enter the extracellular space or bloodstream. Serum levels of S100B increase in patients during the acute phase of brain damage. Over the last decade, S100B has emerged as a candidate peripheral biomarker of blood–brain barrier (BBB) permeability and central nervous system injury.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human S100B protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed
  2. Quintero-Espinosa D, et al. 2021. Mol Neurobiol. . PubMed
RRID
AB_2734531 (BioLegend Cat. No. 676603)
AB_2571890 (BioLegend Cat. No. 676604)

Antigen Details

Structure
92 amino acids with a predicted molecular weight of approximately 10.7 kD.
Distribution

Mainly expressed in mature astrocytes of the brain. It is also found in a variety of other cell types such as T lymphocytes, monocytes, and platelets.

Function
Neurite extension, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly.
Interaction
STK38, CACYBP, ATAD3A, S100A6, CAPZA1 AGER, and PPP5C.
Cell Type
Astrocytes
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Antigen References

1. Wang D, et al. 2008. Prog. Neurobiol. 86:342.
2. Michetti F, et al. 2012. J. Neurochem. 120:644.
3. Zongo D, et al. 2012. Annals. of Emergency Medicine 59:209.
4. Deloulme J, et al. 2000. J. Biol. Chem. 275:35302-10.

Gene ID
6285 View all products for this Gene ID
UniProt
View information about S100B on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/25/2016

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  • Purified anti-S100B
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