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Purified anti-S-Tag Antibody

Pricing & Availability
Clone
BL26881 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
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Product Citations
publications
BL26881_PURE_S-tag_Antibody_1_WB_092216
Recombinant Posi-Tag Epitope Tag Protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-S-Tag (clone BL26881) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • BL26881_PURE_S-tag_Antibody_1_WB_092216
    Recombinant Posi-Tag Epitope Tag Protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-S-Tag (clone BL26881) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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688102 25 µg $89
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Description

S-tag is the name of an oligopeptide derived from pancreatic RNase A. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag. The S-Tag sequence is a fusion peptide tag for recombinant proteins that allows detection by a rapid, sensitive homogeneous assay or by colorimetric detection in Western blots. Proteins can also be rapidly purified from crude extracts using affinity separation method.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic Peptide
Formulation
Liquid in phosphate-buffered solution containing 50% glycerol, 0.25% BSA and 0.02% sodium azide.
Preparation
The antibody was affinity purified from mouse ascites by affinity chromatography using specific immunogen.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Wu L, et al. 2020. Nat Commun. 3.935416667. PubMed
  2. Szymura SJ, et al. 2020. BMC Biol. 18:32. PubMed
RRID
AB_2629593 (BioLegend Cat. No. 688102)

Antigen Details

Structure
The amino acid sequence of the S-tag is: Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser.
Distribution

Derived from pancreatic ribonuclease A (RNase A).

Function
S-tag can be attached to the N- or C-terminus of protein to become S-tag fused protein.
Biology Area
Cell Biology
Antigen References

1. Raines RT, et al. 2000. Methods Enzymol. 326:362.

Gene ID
NA
UniProt
View information about S-Tag on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09/20/2023

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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  • Purified anti-S-Tag
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