Purified anti-Runx3 Antibody

Pricing & Availability
Clone
R3-5G4 (See other available formats)
Other Names
Runt related transcription factor 3, Acute myeloid leukemia 2, Core binding factor α 3 subunit, Polyomavirus enhancer-binding protein 2 α C subunit,
Isotype
Mouse IgG1
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Product Citations
publications
R3-5G4_PURE_Runx3_Antibody_1_050418
Total cell lysates (15 µg protein) from MCF-7 (negative control), HeLa, and Jurkat cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-Runx3 antibody, clone R3-5G4. Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405301) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower) at a 1:2000 dilution. Lane M: MW ladder.
  • R3-5G4_PURE_Runx3_Antibody_1_050418
    Total cell lysates (15 µg protein) from MCF-7 (negative control), HeLa, and Jurkat cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-Runx3 antibody, clone R3-5G4. Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405301) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower) at a 1:2000 dilution. Lane M: MW ladder.
  • R3-5G4_Purified_Runx3_Antibody_2_021920
    Whole cell extracts (180 µg total protein) prepared from Raji cells were immunoprecipitated overnight with 2.5 µg of purified mouse IgG1, κ isotype ctrl antibody (clone MG1-45) (Cat. No. 401402) or purified anti-RUNX3 antibody (clone R3-5G4). The resulting IP fractions and whole cell extract input (5%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit antibody against human RUNX3 overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG (minimal x-reactivity) antibody (Cat. No. 406401) at a 1:3000 dilution. Lane M: Molecular weight marker.
Cat # Size Price Quantity Avail. Save
697901 25 µg $85
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697902 100 µg $215
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Description

The runt related transcription factor (Runx3) is a downstream effector of the TGF-β signaling axis, and has been shown to play a critical function in cancer metastasis through transcriptional control of genes involved in apoptosis, cell signaling, cell migration, and invasion. Additionally, Runx3 plays an essential role in differentiation of CD8 and NK cells. Runx3 forms a heterodimer with the CBFβ subunit. The Runx3-CBFβ complex binds to the core sequence 5'-PYGPYGGT-3' found in enhancer and promoter regions of its target genes, where it can either induce or suppress gene expression. Evidence suggests Runx3 functions as a tumor suppressor in many cancers.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment of human Runx3 (aa: 187-234).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 1.0 µg per mL (1:4000-1:500 dilution). For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes the two isoforms of Runx3 with predicted molecular weights of 44.4 and 45.9 kD.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References

(PubMed link indicates BioLegend citation)
  1. Kim JH, et al. 2014. Oncogenesis. 3: e101. (WB, IP)
  2. Fu Y, et al. 2011. J. Biol. Chem. 13:11803. (WB, IP)
  3. Ito K, et al. 2008. Cancer Cell. 14: 226. (WB, IP)
  4. Yano T, et al. 2006. Mol. Cell Biol. 26: 4474. (WB, EMSA)
  5. Ito K, et al. 2005. Cancer Research. 65: 7743. (WB)
RRID
AB_2687206 (BioLegend Cat. No. 697901)
AB_2687207 (BioLegend Cat. No. 697902)

Antigen Details

Structure
415 and 429 amino acid isoforms with a predicted molecular weight of 44.4 and 45.9 kD, respectively. Isoform 2 has an alternate 19 amino acid in place for the N-terminal 5 amino acids of isoform1. Contains an N-terminal Runt domain, a 120 amino acid region that confers DNA-binding and heterodimerization function, and a proline/serine/threonine rich C-terminal transactivation domain.
Distribution

Cytoplasm, nucleus.

Function
Runx3 facilitates transcriptional regulation of its target genes; functions as tumor suppressor in many types of cancer. Phosphorylation of Runx3 abrogates DNA-binding activity and affects its subcellular localization.
Interaction
Forms a heterodimer with subunit CBFβ. DNA-binding affinity is increased by heterodimerization with CBFβ. Interacts with R-SMADs, TLE1, SRC, FYN, LCK, FOXP3, and ZFHX3.
Antigen References

1. Park J, et al. 2017. Oncotarget. 8:9079.
2. Llorca-Cardenosa MJ, et al. 2016. Oncotarget. 7: 63424.
3. Chuang LS, et al. 2016. Proc. Natl. Acad. Sci. U S A. 113:6490.
4. Ebihara T, et al. 2015. Nat. Immunology. 16:1124.
5. Whittle MC, et al. 2015. Cell. 161:1345.

Gene ID
864 View all products for this Gene ID
UniProt
View information about Runx3 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 02/19/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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