- R3-5G4 (See other available formats)
- Regulatory Status
- Other Names
- Runt related transcription factor 3, Acute myeloid leukemia 2, Core binding factor α 3 subunit, Polyomavirus enhancer-binding protein 2 α C subunit,
- Mouse IgG1
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- Product Citations
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The runt related transcription factor (Runx3) is a downstream effector of the TGF-β signaling axis, and has been shown to play a critical function in cancer metastasis through transcriptional control of genes involved in apoptosis, cell signaling, cell migration, and invasion. Additionally, Runx3 plays an essential role in differentiation of CD8 and NK cells. Runx3 forms a heterodimer with the CBFβ subunit. The Runx3-CBFβ complex binds to the core sequence 5'-PYGPYGGT-3' found in enhancer and promoter regions of its target genes, where it can either induce or suppress gene expression. Evidence suggests Runx3 functions as a tumor suppressor in many cancers.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Purified recombinant fragment of human Runx3 (aa: 187-234).
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IP - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 1.0 µg per mL (1:4000-1:500 dilution). For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody recognizes the two isoforms of Runx3 with predicted molecular weights of 44.4 and 45.9 kD.
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
(PubMed link indicates BioLegend citation)
- Kim JH, et al. 2014. Oncogenesis. 3: e101. (WB, IP)
- Fu Y, et al. 2011. J. Biol. Chem. 13:11803. (WB, IP)
- Ito K, et al. 2008. Cancer Cell. 14: 226. (WB, IP)
- Yano T, et al. 2006. Mol. Cell Biol. 26: 4474. (WB, EMSA)
- Ito K, et al. 2005. Cancer Research. 65: 7743. (WB)
AB_2687206 (BioLegend Cat. No. 697901)
AB_2687207 (BioLegend Cat. No. 697902)
- 415 and 429 amino acid isoforms with a predicted molecular weight of 44.4 and 45.9 kD, respectively. Isoform 2 has an alternate 19 amino acid in place for the N-terminal 5 amino acids of isoform1. Contains an N-terminal Runt domain, a 120 amino acid region that confers DNA-binding and heterodimerization function, and a proline/serine/threonine rich C-terminal transactivation domain.
- Runx3 facilitates transcriptional regulation of its target genes; functions as tumor suppressor in many types of cancer. Phosphorylation of Runx3 abrogates DNA-binding activity and affects its subcellular localization.
- Forms a heterodimer with subunit CBFβ. DNA-binding affinity is increased by heterodimerization with CBFβ. Interacts with R-SMADs, TLE1, SRC, FYN, LCK, FOXP3, and ZFHX3.
- Antigen References
1. Park J, et al. 2017. Oncotarget. 8:9079.
2. Llorca-Cardenosa MJ, et al. 2016. Oncotarget. 7: 63424.
3. Chuang LS, et al. 2016. Proc. Natl. Acad. Sci. U S A. 113:6490.
4. Ebihara T, et al. 2015. Nat. Immunology. 16:1124.
5. Whittle MC, et al. 2015. Cell. 161:1345.
- Gene ID
- 864 View all products for this Gene ID
- View information about Runx3 on UniProt.org
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