Purified anti-RNA Polymerase II RPB3 Antibody

Pricing & Availability
1Y26 (See other available formats)
Regulatory Status
Other Names
RNA polymerase II sub-unit 3, RPB3, POLR2C
Mouse IgG1
Ave. Rating
Submit a Review
Product Citations
Western blot analysis of yeast (saccharomyces cerevisiae) (lane 1) and Jurkat (lane 2). Anti-RNA Polymerase II RPB3 antibody (clone 1Y26) was used and was followed by HRP anti-mouse IgG antibody.
  • 1Y26_Purified_RNAPolymeraseIIRPB3_Antibody_WB_011915
    Western blot analysis of yeast (saccharomyces cerevisiae) (lane 1) and Jurkat (lane 2). Anti-RNA Polymerase II RPB3 antibody (clone 1Y26) was used and was followed by HRP anti-mouse IgG antibody.
Cat # Size Price Quantity Check Availability Save
665004 100 µg $303
Check Availability

Need larger quantities of this item?
Request Bulk Quote

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The RNA polymermase II subunit RPB3 is a component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft and is known to be involved in transcription termination.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Yeast (Saccharomyces cerevisiae)
Antibody Type
Host Species
Recombinant protein
0.22 µm filtered protein solution is in PBS, pH 7.2.
The antibody was purified by affinity chromatography.
1.0 mg/ml
Storage & Handling
Aliquot and store at -20°C. Avoid freeze/thaw cycles.

WB - Quality tested
ELISA - Verified
ChIP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is yeast RPB3-specific and should not be used to detect mammalian RPB3.

Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Svetlov V, et al. 1998. J. Biol. Chem. 273:10827.
Product Citations
  1. Neurohr GE, et al. 2019. Cell. 176:1083. PubMed
  2. Collin P, et al. 2019. Mol Cell. 73:655. PubMed
  3. Yurko N, et al. 2017. Mol Cell. 68:913. PubMed
  4. Jeronimo C, et al. 2019. Cell Rep. 28:1206. PubMed
  5. Ryu HY, et al. 2019. EMBO J. 38:e102003. PubMed
  6. Abraham K, et al. 2016. Nucleic Acids Res. 44: 8870 - 8884. PubMed
AB_2565221 (BioLegend Cat. No. 665004)

Antigen Details

RPB3 is a 45 kD subunit of the yeast RNA polymerase II.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
854791 View all products for this Gene ID
View information about RNA Polymerase II RPB3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/19/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-RNA Polymerase II RPB3

  • Direct-Blot™ HRP anti-RNA Polymerase II RPB3


Remember me
Forgot your password? Reset Password
Request an Account