Purified anti-RGS14 Antibody (Previously Covance catalog# MMS-5248)

Pricing & Availability
Clone
N133/21 (See other available formats)
Other Names
Regulator of G-protein signaling 14, RAP1/RAP2-interacting protein
Previously
Covance Catalog# MMS-5248
Isotype
Mouse IgG2a
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Product Citations
publications
N133.21_Purified_RGS14_IHC_020315.jpg
IHC staining of anti-RGS14 antibody (clone N133/21) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided.
  • N133.21_Purified_RGS14_IHC_020315.jpg
    IHC staining of anti-RGS14 antibody (clone N133/21) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided.
  • N133slash21_PURE_RGS14_Antibody_IHC_2_100517
    IHC staining of anti-RGS14 antibody (clone N133/21) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided.
  • N133slash21_PURE_RGS14_Antibody_WB_100517
    Western blot of anti-RGS14 antibody (clone N133/21). Lane 1: molecular weight marker; Lane 2: 20µg of mouse brain lysate; Lane 3: 20µg of rat brain lysate. Blots were incubated with 10 µg/mL of primary antibody overnight at 4°C, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
821801 100 µl $140.00
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Description

Regulator of G protein Signaling 14 (RGS14) modulates G-protein signaling by binding to G alpha subunits bound to GTP, converting GDP to GTP, and allowing G alpha subunits to bind to G beta/gamma subunits, which are inactive as a G protein.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-544 (full-length) of rat RGS14.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining. 

The optimal working dilution should be determined for each specific assay condition. 
• WB: 5-10 μg/ml
• IHC: 5-10 μg/ml
Tissue: Formalin-fixed Paraffin-embedded tissues 
Pre-treatment: Citrate 
Primary incubation: 24 hours at 4°C

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

*Expected Molecular Weight = 60 kD

WB positive control: Rat and mouse brain membrane lysates. This clone does not cross-react with human RGS14 in WB.

IHC positive control: Rat and mouse brain tissue.

RRID
AB_2564854 (BioLegend Cat. No. 821801)

Related FAQs

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Go To Top Version: 5    Revision Date: 06/06/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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