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Purified anti-RAG2 Antibody

Pricing & Availability
Clone
3G4A26 (See other available formats)
Regulatory Status
RUO
Other Names
V(D)J recombination-activating protein 2
Isotype
Mouse IgG2b, κ
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Product Citations
publications
3G4A26_RAG2_Purified_Antibody_WB_012114.jpg
Western blot analysis of PBMC (lane 1), mouse thymus (lane 2) and Hela (lane 3) cells using anti-RAG2 antibody (3G4A26). GAPDH antibody (poly6314) was used as loading control.
  • 3G4A26_RAG2_Purified_Antibody_WB_012114.jpg
    Western blot analysis of PBMC (lane 1), mouse thymus (lane 2) and Hela (lane 3) cells using anti-RAG2 antibody (3G4A26). GAPDH antibody (poly6314) was used as loading control.
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654902 100 µg $288
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Description

During T cell and B cell development, RAG2 works together with RAG1 to induce DNA rearrangement and assembly of antigen receptor coding segments. Defects in RAG2 gene lead to the complete lack of mature T and B lymphocytes and result in severe combined immunodeficiency (SCID). RAG2 also functions in chromosome binding through its unique plant homeodomain (PHD). Sequestration of recombination signal sequences (RSS) within the nucleosome by RAG2 prevents access by the recombinase complex.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial RAG2 recombinant protein (70-516 aa).
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
Affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5-2.5 µg/ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application

RRID
AB_2561891 (BioLegend Cat. No. 654902)

Antigen Details

Structure
527 amino acids, predicted molecular weight of 59 kD. Containing a core RAG domain with DNA cleavage activity and an atypical PHD domain responsible for binding of trimethylated histone H3 (H3K4me3) and phospholipids.
Distribution
Nucleus.
Function
RAG2 protein acts as a core subunit of the RAG complex, which is responsible for V(D)J recombination of T-cell receptors and immunoglobulins.
Interaction
Interacts with RAG1, HMG1, and HMG2 in V(D)J recombination protein-DNA complexes.
Cell Type
B cells, Tregs
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Neuroscience
Molecular Family
TCRs
Antigen References

1. Fugmann SD, et al. 2000. Annu. Rev. Immunol. 18:495.
2. Oettinger MA. 1992. Trends Genet. 8:413.
3. Hillion S, et al. 2009. Autoimmun. Rev. 8:599.
4. Deriano L, et al. 2011. Nature 471:119.
5. van Til NP, et al. 2012. Mol Ther. 20:1968.
6. Jones JM and Simkus C, et al. 2009. Arch. Immunol. Ther. Exp. 57:105.

Gene ID
5897 View all products for this Gene ID
UniProt
View information about RAG2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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