Purified anti-Rab7A Antibody

Pricing & Availability
Clone
W16034A (See other available formats)
Other Names
Rab7, Ras-Related Protein Rab-7a, Ras-Associated Protein RAB7
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_W16034A_PURE_Rab7A_1_WB_101917.png
Western blot of anti-Rab7A antibody (clone W16034A) and isotype-matched IgG2A control. Blots were incubated with 2µg/ml (left) or IgG2A isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-rat secondary antibody. Enhanced chemiluminescence was used as the detection system. M: Molecular weight marker; brain lysates: 20 µg; recombinant proteins: 10 ng. Purified anti-α-Tubulin (clone AA13) antibody was used as a loading control. Note that the band in the lane loaded with GST-Rab7A proteins in the blot probed with α-tubulin antibody (bottom left) is the remaining signal from the blot probed with anti-Rab7A antibody. Anti-GST antibody was used to confirm that GST-tagged recombinant proteins were loaded in the indicated lanes (data not shown).
  • 1_W16034A_PURE_Rab7A_1_WB_101917.png
    Western blot of anti-Rab7A antibody (clone W16034A) and isotype-matched IgG2A control. Blots were incubated with 2µg/ml (left) or IgG2A isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-rat secondary antibody. Enhanced chemiluminescence was used as the detection system. M: Molecular weight marker; brain lysates: 20 µg; recombinant proteins: 10 ng. Purified anti-α-Tubulin (clone AA13) antibody was used as a loading control. Note that the band in the lane loaded with GST-Rab7A proteins in the blot probed with α-tubulin antibody (bottom left) is the remaining signal from the blot probed with anti-Rab7A antibody. Anti-GST antibody was used to confirm that GST-tagged recombinant proteins were loaded in the indicated lanes (data not shown).
  • 2_W16034A_PURE_Rab7A_Antibody_4_ICC_021418
    ICC staining of purified anti-Rab7A antibody (clone W16034A) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The anti-Rab7A antibody (5 mg/ml) was pre-incubated with or without recombinant human Rab7A or Rab7B proteins (5 μg/ml) for 1 hour at room temperature prior to application to the cells for 24 hours at 4°C. The cells were co-stained with anti-α-Tubulin antibody (5 μg/ml; Cat. 909601; clone AA13), followed by incubation with Alexa Fluor® 594 anti-Rat (Rab7A, red) (Cat. 405422) and Alexa Fluor® 488 anti-mouse (α-Tubulin, green) (Cat. 405326) secondary antibodies for 1 hour at room temperature. Nuclei were counterstained with DAPI. Images were captured with a 40X objective.
  • 3_W16034A_PURE_Rab7A_2_IF_101917.png
    ICC staining of anti-Rab7A antibody (clone W16034A) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The anti-Rab7A antibody (5 µg/ml) was pre-incubated with or without recombinant human Rab7A or Rab7B proteins (5 µg/ml) for one hour at room temperature prior to application to the cells for 24 hours at 4°C. The cells were co-stained with anti-α-Tubulin antibody (5 µg/ml; clone AA13), followed by incubation with Alexa Fluor® 594 anti-Rat (Rab7A, red) and Alexa Fluor® 488 anti-mouse (α-Tubulin, green) secondary antibodies for one hour at room temperature. Nuclei were counterstained with DAPI. Images were captured with a 40X objective. Scale bar: 50 µm.
  • 4_W16034A_PURE_Rab7A_3_IHC_101917.png
    IHC staining of anti-Rab7A antibody (clone W16034A) on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with the primary antibody at 5 µg/ml overnight at 4°C. Tissues were incubated with DAB for twenty minutes followed by hematoxylin counterstaining. Images were taken using 40X objectives.
  • 5_W16034A_PURE_Rab7A_Antibody_ELISA_071317
    Direct ELISA of anti-Rab7A antibody (clone W16034A) binding to plate-immobilized recombinant human Rab7A, Rab7B, or PBS. ELISA was performed by coating wells with 150 ng of each recombinant protein for two hours at 37°C. Anti-Rab7A antibody was serially diluted and added to the plate with the concentration indicated on the x-axis, followed by incubation at 37°C for one hour. The plates were then incubated with horseradish peroxidase labeled goat anti-rat secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine) was used as the detection system.
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Cat # Size Price Quantity Avail. Save
850401 25 µg $105
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850402 100 µg $265
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Description

Rab7, a small GTPase of the Rab family, plays multiple roles including vesicular transport, endocytic trafficking, and autophagy. Rab7 is associated with both the endosome and lysosome, and it facilitates endosomal maturation, transport from the late endosome to the lysosome, and positioning of the endosome and lysosome via regulating their movement along cytoskeleton. Consistently, mutations or dysfunctions of Rab7 result in traffic disorders, which cause various diseases, such as neuropathy, cancer and lipid metabolism disease. Rab7 also plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full-length recombinant human Rab7A protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Direct ELISA, ICC, IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 µg per ml. For immunocytochemistry, the suggested usage is 1.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 µg per ml.  It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2715874 (BioLegend Cat. No. 850401)
AB_2715875 (BioLegend Cat. No. 850402)

Antigen Details

Structure
Rab7A is a 207 amino acid protein with a molecular mass of 23 kD.
Distribution

Tissue distribution: Rab7A is ubiquitously expressed.
Cellular distribution: Plasma membrane, cytosol, lysosome, endosome, and Golgi apparatus.

Function
Rab7A is involved in the endocytic pathway and autophagy.
Interaction
Rab7A interacts with RILP, Rab1A, Rab11A, and UBC.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Autophagosome Markers
Antigen References

1. Guerra F, Bucci C. 2016. Cells. 5(3).

Gene ID
7879 View all products for this Gene ID
UniProt
View information about Rab7A on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/18/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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