Purified anti-PSD-93 (Chapsyn-110) Antibody (Previously Covance catalog# MMS-5129)

Pricing & Availability
Clone
N18/28 (See other available formats)
Other Names
Disks large homolog 2, chapsyn-110, discs large homolog 2, synaptic density protein PSD-93, postsynaptic density protein PSD-93, channel-associated protein of synapse-110, PSD93
Previously
Covance Catalog# MMS-5129
Isotype
Mouse IgG2a
Ave. Rating
0 reviews
N18slash28_PURE_PSD-93_Antibody_1_WB_021218.jpg
Western blot of purified anti-PSD-93 (Chapsyn-110) antibody (clone N18/28). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20ug of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N18slash28_PURE_PSD-93_Antibody_1_WB_021218.jpg
    Western blot of purified anti-PSD-93 (Chapsyn-110) antibody (clone N18/28). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20ug of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N18slash28_PURE_PSD-93_Antibody_2_IHC-P_021218.jpg
    IHC staining of purified anti-PSD-93 (Chapsyn-110) antibody (clone N18/28) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N18slash28_PURE_PSD-93_Antibody_3_IHC-P_021218.jpg
    IHC staining of anti-PSD-93 (Chapsyn-110) antibody (clone N18/28) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
818301 100 µl $140.00
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Description

PSD-93, also known as DLG2 or Chapsyn-110, is a member of the MAGUK family important in the scaffolding of receptors with adhesion molecules on the postsynaptic membrane.  It regulates surface expression of receptors by directly interacting with the cytoplasmic tail of NMDA receptor subunits and inward rectifying potassium channels, and is involved in the regulation of synaptic stability at synapses.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a full-length fusion protein (amino acids 1-852) of rat Chapsyn-110 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunohistochemistry, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).

This antibody is less reactive in human.

The antibody does not cross-react wtih SAP97, SAP102, or PSD-95.

RRID
AB_2564819 (BioLegend Cat. No. 818301)

Related FAQs

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Go To Top Version: 2    Revision Date: 07/01/2016

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