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Purified anti-Prox1 Antibody

Pricing & Availability
Clone
W16098A (See other available formats)
Regulatory Status
RUO
Other Names
Prospero homeobox protein 1, Homeobox prospero-like protein PROX1,
Isotype
Rat IgG2a, κ
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Product Citations
publications
W16098A_PURE_Prox1_Antibody_051017
Total cell lysates (15 µg protein) from HeLa (negative control), HepG2 and NIH3T3 cells were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-Prox1 (clone W16098A) antibody (upper) or loading control anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for anti-Prox1 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH antibody. Lane M: molecular weight ladder.
  • W16098A_PURE_Prox1_Antibody_051017
    Total cell lysates (15 µg protein) from HeLa (negative control), HepG2 and NIH3T3 cells were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-Prox1 (clone W16098A) antibody (upper) or loading control anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for anti-Prox1 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH antibody. Lane M: molecular weight ladder.
  • W16098A_PURE_Prox1_Antibody_2_020619
    HepG2 cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 0.5 µg/ml anti- Prox1 antibody (clone W16098A) overnight at 4° followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG for one hour at room temperature. Actin filaments were labeled with Flash Phalloidin™ Green 488. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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696902 100 µg $282
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Description

Prospero homeobox protein 1 (PROX1) is a homeobox transcription factor responsible for progenitor cell differentiation and the development of several organs, such as the central nervous system, lymphatics, pancreas, heart, lens, skeletal muscle, and liver. Prox1-deficient mice lack lymphatic systems and die at day 14.5 of embryogenesis due to multiple developmental defects. In addition to its role in physiological development, PROX1 is involved in carcinogenesis and can act either as a tumor suppressor or as an oncogene depending on the tissue and its expression level. Prox1 is also implicated in the regulation of metabolism in the liver by regulating activities of nuclear receptors including hepatocyte nuclear factor 4a (HNF4a) and liver receptor homolog-1 (LRH-1).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Prox1 recombinant protein (2-259 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 0.5 - 5 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. 

Application Notes

This clone does not cross react with mouse (in-house tested).

RRID
AB_2687107 (BioLegend Cat. No. 696902)

Antigen Details

Structure
737 amino acids with a predicted molecular weight of 83 kD. Contains a C-terminal Prospero-type homeo domain responsible for DNA binding.
Distribution

Nucleus.

Function
PROX-1 is a homeobox transcription factor involved in the embryonic development and carcinogenesis.
Interaction
Interacts with RORA, RORC, and HNF4a.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Kivelä R, et al. 2016. Nat. Commun. 7:13124.
2. Stergiopoulos A, et al. 2015. Front Cell Neurosci. 8:454.
3. Ragusa S, et al. 2014. Cell Rep. 8:1957.
4. Elsir T, et al. 2012. Cancer Metastasis Rev. 31:793.
5. Karalay O, et al. 2011. Proc. Natl. Acad. Sci. USA. 108:5807.
6. Johnson NC, et al. 2008. Genes Dev. 22:3282.

Gene ID
5629 View all products for this Gene ID
UniProt
View information about Prox1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/12/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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