Purified anti-Presenilin 1 (N terminus) Antibody (Previously Covance catalog# SIG-39194)

Pricing & Availability
Clone
NT1 (See other available formats)
Other Names
AD3, FAD, PS-1, S182, presenilin-1, gamma-secretase
Previously
Covance Catalog# SIG-39194
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
NT1_Purified_Presenilin-1_Antibody_1_072318
Western blot of purified anti-Presenilin 1 (N terminus) antibody (clone NT1). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain cerebellum membrane lysate; Lane 3: 40 µg of human brain cortex membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NT1_Purified_Presenilin-1_Antibody_1_072318
    Western blot of purified anti-Presenilin 1 (N terminus) antibody (clone NT1). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain cerebellum membrane lysate; Lane 3: 40 µg of human brain cortex membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NT1_Purified_Presenilin-1_Antibody_2_072318
    Western blot of purified anti-Presenilin 1 (N terminus) antibody (clone NT1). Lane 1: Molecular weight marker; Lane 2: 40 µg of HeLa lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
823404 25 µl $95
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823401 200 µl $427
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Description

Presenilin 1 is one of two transmembrane related-proteins, the other being Presenilin 2, that form the catalytic subunits of the gamma-secretase intramembrane protease complex. The gamma secretase complex catalyzes cleavage of a number of integral membrane proteins including Amyloid precursor protein (APP), Notch, Erb4 and E-cadherin.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BioLegend's mouse anti-Presenilin 1 was raised against a portion of the n-terminal region of Presenilin 1 (RRSLGHPEP).
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is against the N-terminus of Presenilin 1 and detects the N-terminal fragment (NTF) generated from the proteolysis of the full-length protein (52kD).

Application References

(PubMed link indicates BioLegend citation)
  1. Mathews PM, et al. 2000. Mol Med. 6(10)
  2. Cohen RM, et al. 2013. J. Neurosci. 15:6245. (WB) PubMed
  3. Laudon H, et al. 2004. J Neurochem. 89:44-53
  4. Ahn K, et al. 2010. Proc Natl Acad Sci U S A. 107:21435
Product Citations
  1. Cohen R, et al. 2013. J Neurosci. 33:6245-6256. PubMed
Publication Library
RRID
AB_2734591 (BioLegend Cat. No. 823404)
AB_2564868 (BioLegend Cat. No. 823401)

Antigen Details

Structure
Expected MW: 26 kD
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Secretases
Gene ID
5663 View all products for this Gene ID
UniProt
View information about Presenilin 1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 07/23/2018

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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