- Poly6327 (See other available formats)
- Other Names
- B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, p94
- Rabbit Polyclonal IgG
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- Product Citations
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The Raf proteins are a family of serine/threonine-specific kinases that serve as a central intermediate in transmitting extracellular signals to the mitogen-activated protein kinase cascade, which controls cell growth, differentiation and survival. Three isoforms of Raf proteins have been found in mammalian cells: Raf-1, A-Raf and B-Raf. The B-raf involved in signal transduction from the membrane to the nucleus. It has been reported that T598 and S601 are the major phosphorylation sites of B-Raf in response to oncogenic Ras, and phosphorylation of these two residues is required for full activation of B-Raf. Mutations in the B-Raf gene have been reported in a number of human cancers, including malignant melanoma, thyroid cancer, and colorectal carcinoma. The poly6327 antibody recognizes human phosphorylated B-Raf (Thr598/Ser601) and has been shown to be useful for immunoflorence staining.Product Details
- Antibody Type
- Host Species
- Modified synthetic peptide FGLATVKSRWSGS
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
- The antibody was purified by antigen-affinity chromatography.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Upon receipt, store frozen at -20°C.
ICC - Quality tested
IHC-P - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution range of 1:100 - 1:400 is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a dilution of 1:100 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This product may contain other non-IgG subtypes.
AB_2191774 (BioLegend Cat. No. 632702)
- Belongs to the Raf/mil family of serine/threonine protein kinases, predicted molecular weight 84 kD
- B-Raf involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It has been reported that T598 and S601 are the major phosphorylation sites of B-Raf in response to oncogenic Ras, and phosphorylation of these two residues is
- Biology Area
- Cell Biology
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
1. Avruch J, et al. 1994. Trends Biochem Sci. 19:279.
2. Brose MS, et al. 2002. Cancer Res. 62:6997.
3. Zhang B-H, et al. 2000. EMBO 19:5429.
- Gene ID
- 673 View all products for this Gene ID
- View information about Phosphorylated B-Raf on UniProt.org
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