- Poly6494 (See other available formats)
- Other Names
- Eukaryotic translation initiation factor 2 alpha kinase 3, PRKR-like endoplasmic reticulum kinase, heme-regulated EIF2-alpha kinase
- Rabbit Polyclonal IgG
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- Product Citations
|Cat #||Size||Price||Quantity Avail.||Save|
|649401||100 µl (5 Western blots)||$125|
|649402||400 µl (20 Western blots)||$350|
PERK (protein kinase R-like endoplasmic reticulum kinase) is a type I membrane protein located in the endoplasmic reticulum (ER). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress. PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain. Phosphorylation of PERK at Ser713 can be used as a marker for its activation status. The predicted phosphorylation site and surrounding residues of Ser713 are: HIEIIAPS*PQRSRSF.Product Details
- Antibody Type
- Host Species
- Modified synthetic peptide
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store at -20°C.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 20 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The optimal concentration should be determined by titration for each individual assay of interest.
This product may contain other non-IgG subtypes.
(PubMed link indicates BioLegend citation)
- Product Citations
AB_10642966 (BioLegend Cat. No. 649401)
AB_10640071 (BioLegend Cat. No. 649402)
- 1115 amino acids; 125 kD predicted. The observed MW for phosphor-PERK is approximately 150-170 kD.
Endoplasmic reticulum membrane; Single-pass type I membrane protein
- Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.
- Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells.
- Biology Area
- Cell Biology, Protein Synthesis, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
1. Shi Y, et al. 1998. Mol. Cell. Biol. 18:7499.
2. Shi Y, et al. 1999. J. Biol. Chem. 274:5723.
3. Cybulsky AV, et al. 2005. J. Biol. Chem. 280:24396.
4. Kohno K. 2010. J. Biochem. 147:27.
- Gene ID
- 9451 View all products for this Gene ID
- View information about PERK Phosphorylated on UniProt.org
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