- 5A5 (See other available formats)
- Regulatory Status
- Other Names
- Poly (ADP-ribose) polymerase
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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PARP (Poly (ADP-ribose) polymerase) is a 113 kD nuclear protein that can exist as a homo- or hetero-dimer. This protein acts as a molecular "nick sensor" and functions in base excision repair, poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism and participates in protein modification to enhance or repress transcription. PARP is ribosylated by PARP2 and is a target for caspase cleavage during apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3. The 5A5 monoclonal antibody recognizes the N-terminal region of human and mouse PARP and has been shown to be useful for Western blotting and immunofluorescence staining.Product Details
- Antibody Type
- Host Species
- Recombinant (partial), N-terminal 2/3 sequence of PARP
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
WB - Quality tested
KO/KD-WB, ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL (1:500-1:1000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody recognizes N-terminal short cleaved PARP after Staurosporine treatment at around 24kDa.
(PubMed link indicates BioLegend citation)
- Product Citations
AB_2160595 (BioLegend Cat. No. 614301)
AB_2299318 (BioLegend Cat. No. 614302)
- PARP family, BRCT domain, homo- or hetero-dimer; 113 kD
- Molecular "nick sensor"; base excision repair, catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture, DNA metabolism; protein modification may enhance or repress transcription
- Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modifies TATA-BP, YY1, Sp1, NF-B, p53 and others
- Ribosylation by PARP2
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, DNA Repair/Replication, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Cherney B, et al. 1987. P. Natl. Acad. Sci. USA 84:8370.
2. Ikejima M, et al. 1990. J. Biol. Chem. 265:21907.
3. Ying W, et al. 2001. P. Natl. Acad. Sci. USA 98:12227.
4. Noel G, et al. 2003. BMC Cell Biol. 4:7.
- Poly(ADP-ribose) glycohydrolase removes ribose chains, allows quick, transient ribosylation of proteins
- Gene ID
- 142 View all products for this Gene ID
- View information about PARP on UniProt.org
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