Purified anti-PARP Antibody

Pricing & Availability
Clone
5A5 (See other available formats)
Regulatory Status
RUO
Other Names
Poly (ADP-ribose) polymerase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5A5_PURE_PARP_Antibody_1_WB_102317
Western blot analysis of cell lysates from HeLa cells, untreated (-) or treated with Staurosporine (+, 1 µM, 8 hours), using PARP (5A5) Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (#405306). Direct-Blot™ HRP anti-β-actin (#643807) was used as a loading control.
  • 5A5_PURE_PARP_Antibody_1_WB_102317
    Western blot analysis of cell lysates from HeLa cells, untreated (-) or treated with Staurosporine (+, 1 µM, 8 hours), using PARP (5A5) Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (#405306). Direct-Blot™ HRP anti-β-actin (#643807) was used as a loading control.
  • 5A5_Pure_PARP_Antibody_2_042314
    Total lysates (15 µg protein) from 293T and 293T/PARP knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-PARP antibody, clone 5A5 (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • 5A5_Pure_PARP_Antibody_3_042314
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of purified PARP (clone 5A5) (red) in blocking buffer overnight at 4°C and followed by anti-mouse IgG Dylight™ 594 staining for 2 hours and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C . Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
Cat # Size Price Quantity Avail. Save
614301 25 µg $85.00
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614302 100 µg $185.00
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Description

PARP (Poly (ADP-ribose) polymerase) is a 113 kD nuclear protein that can exist as a homo- or hetero-dimer. This protein acts as a molecular "nick sensor" and functions in base excision repair, poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism and participates in protein modification to enhance or repress transcription. PARP is ribosylated by PARP2 and is a target for caspase cleavage during apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3. The 5A5 monoclonal antibody recognizes the N-terminal region of human and mouse PARP and has been shown to be useful for Western blotting and immunofluorescence staining.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant (partial), N-terminal 2/3 sequence of PARP
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL (1:500-1:1000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes N-terminal short cleaved PARP after Staurosporine treatment at around 24kDa.

Application References

(PubMed link indicates BioLegend citation)
  1. Bogiatzi SI, et al. 2012. J Allergy Clin Immunol. 130:233. PubMed.
  2. Gu Y, et al. 2013. Acta Biochim Biophys Sin. PubMed
Product Citations
  1. Nakaya M, et al. 2017. J Clin Invest. 127:383. PubMed
  2. Bogiatzi S, et al. 2012. J Allergy Clin Immunol. 130:233. PubMed
  3. Gu Y, et al. 2013. Acta Biochim Biophys Sin. 45:904. PubMed
  4. Wang F, et al. 2020. Int J Oncol. 57:707. PubMed
RRID
AB_2160595 (BioLegend Cat. No. 614301)
AB_2299318 (BioLegend Cat. No. 614302)

Antigen Details

Structure
PARP family, BRCT domain, homo- or hetero-dimer; 113 kD
Distribution

Nuclear

Function
Molecular "nick sensor"; base excision repair, catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture, DNA metabolism; protein modification may enhance or repress transcription
Interaction
Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modifies TATA-BP, YY1, Sp1, NF-B, p53 and others
Modification
Ribosylation by PARP2
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, DNA Repair/Replication, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Cherney B, et al. 1987. P. Natl. Acad. Sci. USA 84:8370.
2. Ikejima M, et al. 1990. J. Biol. Chem. 265:21907.
3. Ying W, et al. 2001. P. Natl. Acad. Sci. USA 98:12227.
4. Noel G, et al. 2003. BMC Cell Biol. 4:7.

Regulation
Poly(ADP-ribose) glycohydrolase removes ribose chains, allows quick, transient ribosylation of proteins
Gene ID
142 View all products for this Gene ID
UniProt
View information about PARP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 8    Revision Date: 09/30/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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