Purified anti-Pan-Cytokeratin Antibody

Pricing & Availability
Clone
AE-1/AE-3 (See other available formats)
Other Names
Pan-Cytokeratin (AE-1/AE-3), AE1/AE3
Isotype
Mouse IgG1, κ
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Product Citations
publications
AE-1slashAE-3_PURE_Cytokeratin_Antibody_IHC-P_1_103118
IHC staining of purified anti-Pan-Cytokeratin antibody (clone AE-1/AE-3) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 1 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, AEC, Cat. No. 929401) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • AE-1slashAE-3_PURE_Cytokeratin_Antibody_IHC-P_1_103118
    IHC staining of purified anti-Pan-Cytokeratin antibody (clone AE-1/AE-3) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 1 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, AEC, Cat. No. 929401) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • AE-1slashAE-3_PURE_Cytokeratin_Antibody_IHC-P_2_103118
    Formalin-fixed paraffin-embedded human tonsil treated with a citrate buffer pH6 and heat for antigen retrieval was stained with purified Pan-Cytokeratin clone AE-1/AE-3, conjugated and detected with a Cy3 conjugated CODEX™ oligonucleotide duplex (red). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Cat # Size Price Quantity Avail. Save
914204 100 µg $195
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Description

AE-1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with molecular weights of 56.5, 50, 48 and 40 kD. AE-3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including cytokeratins with molecular weights of of 64, 59, 58, 56, and 52 kD.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, Cross-Reactivity: Dog, Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using human epidermal keratins.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Kou R, et al. 2018. Oncol Rep. 7:2013. PubMed
  2. Chang TH, et al. 2020. Cell Death Dis. 11:338. PubMed
  3. Xie S, et al. 2020. Ann Transl Med. 1.384027778. PubMed
  4. Tiago M, et al. 2020. Br J Cancer. 122:789. PubMed
RRID
AB_2616960 (BioLegend Cat. No. 914204)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
NA
UniProt
View information about Pan-Cytokeratin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/10/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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