Purified anti-p53 Antibody

Pricing & Availability
Clone
BP53-12 (See other available formats)
Other Names
Tumor protein p53 (TP53)
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
BP53-12_Purified_p53_Antibody_031519_updated.png
Whole cell extracts (15 µg total protein) from HEK293, A431, and THP-1 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to PVDF, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-p53 Antibody, clone BP53-12, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in descending of predicted p53 expression; Predicted expression data was obtained from Human Protein Atlas.
  • BP53-12_Purified_p53_Antibody_031519_updated.png
    Whole cell extracts (15 µg total protein) from HEK293, A431, and THP-1 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to PVDF, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-p53 Antibody, clone BP53-12, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in descending of predicted p53 expression; Predicted expression data was obtained from Human Protein Atlas.
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628201 25 µg $85
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628202 100 µg $185
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Description

p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.

Product Details
Technical data sheet

Product Details

Reactivity
Human, non-human primates
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acid: 20-25 of human p53
Formulation
This antibody is provided in Tris-buffered solution, pH 8.0, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
This antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Terada T, et al. 1994. Mod. Pathol. 7:249. (IHC)
  2. Lanoix D, et al. 2013. Mol Cell Endocrinol. 381:35. PubMed
Product Citations
  1. Mandriota S, et al. 2010. J Biol Chem. 285:13092. PubMed
  2. Lanoix D, et al. 2013. Mol Cell Endocrinol. 381:35. PubMed
Publication Library
RRID
AB_439766 (BioLegend Cat. No. 628201)
AB_439767 (BioLegend Cat. No. 628202)

Antigen Details

Structure
Tetramer; 53 kD
Distribution

Nuclear when activated

Function
Tumor suppressor, transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, cell adhesion
Interaction
mdm2, mdmx, TopoI, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, HDAC1-3; mutant p53 associates with p63, p73
Modification
Phosphorylation, Acetylation, Ribosylation, Ubiquitination, Sumoylation
Cell Type
Mesenchymal Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Stem Cells, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Vogelstein B, et al. 1992. Cell. 70:523.
2. Shieh S, et al. 1997. Cell. 91:325.
3. Mihara M, et al. 2003. Mol. Cell 11:577.
4. Saito S, et al. 2003. J. Biol. Chem. 278:37536.

Regulation
Ubiquinated for degradation via mdm2. Upregulated by genotoxic or other cellular stresses
Gene ID
7157 View all products for this Gene ID
UniProt
View information about p53 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03/15/2019

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