Purified anti-NSE Antibody (Previously Covance catalog# MMS-518P)

Pricing & Availability
Clone
NSE-P1 (See other available formats)
Other Names
Gamma-enolase, neural enolase, neuron-specific enolase, 2-phospho-D-glycerate hydrolyase, ENO2, NSE
Previously
Covance Catalog# MMS-518P
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2_NSE-P1_PURE_NSE_Antibody_1_01318
IHC staining of purified anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 488 goat anti-mouse IgG for two hour at room temperature. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2_NSE-P1_PURE_NSE_Antibody_1_01318
    IHC staining of purified anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 488 goat anti-mouse IgG for two hour at room temperature. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_NSE-P1_PURE_NSE_Antibody_2_01318
    IHC staining of purified anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG for two hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective.
  • NSE-P1_Purified_NSE_Antibody_3_010319.png
    Western blot of purified anti-NSE antibody (clone NSE-P1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. Lane 3: 20 µg of mouse brain lysate. Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426303) was used as the detection system.
Cat # Size Price Quantity Avail. Save
804908 25 µl $95
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804901 200 µl $235
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Description

Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers. Expression of these isozymes can be cell-type and development-specific. This gene encodes gamma enolase (enolase 2) and is expressed in mature neurons and cells of neuronal origin. The alpha/alpha homodimers are expressed in embryo and in most adult tissues. The alpha/beta heterodimers and the beta/beta homodimers are found in striated muscle, and the alpha/gamma heterodimers and the gamma/gamma homodimers are expressed in neurons. Neuron specific enolase is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acids 416-433. It recognizes the sequence LGDEARFAGHNFRNPSVL.
Formulation
Phosphate-buffered solution + 0.03% thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Validated
ELISA - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The reactivity of Alexa Fluor® 647 and Alexa Fluor® 488 anti-NSE antibody was validated in FFPE mouse and rat tissue.

Additional reported applications (for the relevant formats) include: ELISA2 and immunohistochemistry1,3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Yoo J, et al. 2002. Arch. Pathol. Lab Med. 126(8):979. (IHC-P)
  2. Duncan ME, et al. 1992. J. Immuno. Methods. 151:227 (ELISA)
  3. Chetty R. 2000. Pathology. 32(3):209. (IHC-P)
  4. Murray GI, et al. 1993. J. Clin. Pathol. 46:993. (IHC-P)
RRID
AB_2728530 (BioLegend Cat. No. 804908)
AB_2564673 (BioLegend Cat. No. 804901)

Antigen Details

Structure
Gamma enolase is a 434 amino acid protein with a molecular mass of 47 kD.
Distribution

Tissue distribution: Nervous and musculoskeletal systems.

Cellular distribution: Secreted, cytosol, plasma membrane, cytoskeleton, and nucleus.

Function
Gamma enolase has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Phospho-Proteins
Antigen References
  1. Muoio B, et al. 2017. Int J Biol Markers. 10.5301/ijbm.5000286.
  2. Huang L, et al. 2017. Oncotarget. 8(38): 64358.
  3. Isgro MA, et al. 2015. Adv Exp Med Biol. 867:125.
  4. Zaheer S, et al. 2013. Ann Indian Acad Neurol. 16(4):504.
Gene ID
2026 View all products for this Gene ID
UniProt
View information about NSE on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/03/2019

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