Purified anti-NPM/B23-Phosphorylated (Thr234/Thr237) Antibody

Pricing & Availability
Clone
Poly6191 (See other available formats)
Other Names
Numatrin, nucleophosmin 1, nucleolar phosphoprotein B23
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
NPMB23Thr234237_091004
Hela cells were treated with 300 µM mimosine for 16 h, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18h. Nuclear extract was western blotted with Poly6191. In addition to the specific NPM/B23 band, this antibody recognizes several other non-specific proteins with molecular weights >66kD.
  • NPMB23Thr234237_091004
    Hela cells were treated with 300 µM mimosine for 16 h, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18h. Nuclear extract was western blotted with Poly6191. In addition to the specific NPM/B23 band, this antibody recognizes several other non-specific proteins with molecular weights >66kD.
  • Poly6191_IHC_062508
    Formalin-fixed paraffin-embedded human epidermis tissue was stained with Poly6191 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Cat # Size Price Quantity Avail. Save
619101 50 µl (5 Western blots) $125
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619102 200 µl (20 Western blots) $350
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Description

NPM/B23 (also known as numatrin, nucleophosmin 1, nucleolar phosphoprotein B23) is a member of the nucleophosmin/nucleoplasmin family that shuttles between the nucleus and cytoplasm. This protein regulates the stability and transcriptional activity of p53 and acts as a molecular chaperone. NPM/B23 preferentially binds to denatured proteins and has been shown to stimulate DNA polymerase activity and control the duplication of centrosomes. The chaperone activity of NPM/B23 is regulated by protein kinase CK2 and promotes release of denatured substrates from NPM/B23. NPM/B23 is modified by phosphorylation onThr199. This protein has been shown to bind to RNA and DNA and interact with nucleolar proteins including nucleolin, protein P120, HIV-1 Rev protein, and hepatitis delta antigens. The Poly6191 antibody recognizes human phosphorylated NPM/B23 (Thr234/Thr237) and has been shown to be useful for Western blotting.

Product Details
Technical data sheet

Product Details

Reactivity
Human, reacts against Thr234/Thr237-phosphorylated NPM/B23
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Modified peptide
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol. Bovine serum albumin is present at 1 mg/ml final concentration (USA origin).
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
IHC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Wang Z, et al. 2010. Sci Signal. 3;2. PubMed
Product Citations
  1. Olson S 2006. J Cell Sci. 119:3676. PubMed
  2. Wang Z, et al. 2010. Sci Signal. 3:ra2. PubMed
Publication Library
RRID
AB_2155168 (BioLegend Cat. No. 619101)
AB_2155167 (BioLegend Cat. No. 619102)

Antigen Details

Structure
Nucleophosmin/nucleoplasmin family, member 1
Distribution
Nucleolar protein, can shuttle between nucleus and cytoplasm
Function
Regulates the stability and transcriptional activity of p53, acts as a molecular chaperone. Preferentially binds to denatured proteins. Has been shown to stimulate DNA polymerase α activity and control the duplication of centrosomes
Interaction
Binds to RNA, DNA, and interacts with nucleolar proteins including nucleolin, protein P120, HIV-1 Rev protein, and hepatitis delta antigens
Modification
Phosphorylation
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Szebeni A, et al. 2003. J. Biol. Chem. 278:9107.
2. Colombo E, et al. 2002. Nat. Cell Biol. 4:529.
3. Chan PK, et al. 1986. J. Biol. Chem. 261:14335.

Regulation
Chaperone activity is regulated by protein kinase CK2, promotes release of denatured substrates from NPM/B23
Gene ID
4896 View all products for this Gene ID
UniProt
View information about NPM/B23-Phosphorylated on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

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