Purified anti-Notch 1 Antibody (Previously Covance catalog# MMS-5169)

Pricing & Availability
Clone
N253/32 (See other available formats)
Other Names
Neurogenic locus notch homolog protein 1, mT14, p300, Motch A, Notch gene homolog 1, major type A protein, transmembrane receptor Notch1, N1, Mis6, Tan1, lin-12, 9930111A19Rik
Previously
Covance Catalog# MMS-5169
Isotype
Mouse IgG1
Ave. Rating
0 reviews
N253slash32_Purified_Notch_1_Antibody_IHC_030415
IHC staining of purified anti-NOTCH 1 antibody (clone N253/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • N253slash32_Purified_Notch_1_Antibody_IHC_030415
    IHC staining of purified anti-NOTCH 1 antibody (clone N253/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • N253slash32_Purified_Notch_1_Antibody_NPRF_022318
    Western blot of purified anti-NOTCH 1 antibody (clone N253/32). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain frontal cortex membrane; Lane 3: 30 µg of mouse brain membrane lysate; Lane 4: 30ug of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
819101 100 µl $140.00
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Description

Notch 1, also known as TAN-1, is a transmembrane protein. Its extracellular domain contains 29 epidermal growth factor-like (EGF) repeats and 3 Lin/Notch Glp (LNR) repeats, the intracellular domain contains 5 CDC10/Ankryn repeats (ANK), 1 proline, glutamate, serine, threonine-rich (PEST) motif, and 1 regulation of amino acid metabolism 23 (RAM23) domain. Notch 1 regulates the development, differentiation, and survival of a broad spectrum of cell lineages. It is involved in myogenesis, neurogenesis, gliogenesis, and lymphocyte development, resulting in Notch 1 expression in many organs such as brain, lung, thymus, spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart. Notch 1 ligands are Jagged 1, Jagged 2, Delta 1, and Delta 4. Upon ligand binding, the intracellular domain of Notch 1 is cleaved and translocates to the cell nucleus where it forms a transcriptional activator complex with RBP-J κ.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P). This antibody is less reactive in human tissue in IHC-P.

RRID
AB_2564827 (BioLegend Cat. No. 819101)

Antigen Details

Structure
Expected molecular weight: >270 kD, 120 kD
Gene ID
18128 View all products for this Gene ID
UniProt
View information about Notch 1 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 02/16/2016

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