Purified anti-NMDAR2B Antibody (Previously Covance catalog# MMS-5149)

Pricing & Availability
Clone
N59/20 (See other available formats)
Other Names
GluN2B/NR2B glutamate receptor, Glutamate receptor ionotropic, NMDA 2B, NR2B, NMDAR2B, glutamate receptor, ionotropic, NMDA2B, N-methyl D-aspartate receptor subtype 2B, glutamate [NMDA] receptor subunit epsilon-2
Previously
Covance Catalog# MMS-5149
Isotype
Mouse IgG2a
Ave. Rating
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Product Citations
publications
N59-20_PURE_NMDAR2B_WB_113018
Western blot of purified anti-NMDAR2B antibody (clone N59/20). Lane 1: Molecular weight marker; Lane 2: 5 µg of mouse brain membrane lysate; Lane 3: 10 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N59-20_PURE_NMDAR2B_WB_113018
    Western blot of purified anti-NMDAR2B antibody (clone N59/20). Lane 1: Molecular weight marker; Lane 2: 5 µg of mouse brain membrane lysate; Lane 3: 10 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
832501 100 µl $140.00
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Description

Glutamate activated Ion channels that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDA receptor plays an essential role in the induction of LTP in the CA1 and dentate areas of the hippocampus as the specific NMDA antagonist, APV blocks LTP in these areas. This receptor has also been linked to neuronal development and it has been implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 20-271 (extracellular, N-terminus) of rat GluN2B/NR2B glutamate receptor protein.
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

RRID
AB_2564957 (BioLegend Cat. No. 832501)

Related FAQs

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Go To Top Version: 3    Revision Date: 11/30/2018

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