Purified anti-Nicastrin Antibody

Pricing & Availability
Clone
9C3 (See other available formats)
Other Names
NCSTN
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
9C3_PURE_Nicastrin_Antibody_1_WB_032318
Western blot of anti-Nicastrin antibody (clone 9C3) and isotype-matched control mouse IgG2b. Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blots were incubated with 0.5 μg/mL of anti-Nicastrin or mouse IgG overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. In human the antibody also detects the deglycosylated nicastrin.
  • 9C3_PURE_Nicastrin_Antibody_1_WB_032318
    Western blot of anti-Nicastrin antibody (clone 9C3) and isotype-matched control mouse IgG2b. Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blots were incubated with 0.5 μg/mL of anti-Nicastrin or mouse IgG overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. In human the antibody also detects the deglycosylated nicastrin.
  • 9C3_PURE_Nicastrin_Antibody_2_IHC-P_032318
    IHC staining of anti-Nicastrin antibody (clone 9C3) on formalin-fixed paraffin-embedded human breast cancer tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 9C3_PURE_Nicastrin_Antibody_3_IHC-P_032318
    IHC staining of anti-Nicastrin antibody (clone 9C3) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 9C3_PURE_Nicastrin_Antibody_4_IHC-P_032318
    IHC staining of anti-Nicastrin antibody (clone 9C3) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
852301 25 µg $95
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852302 100 µg $235
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Description

Nicastrin is one of the essential components of the γ-secretase complex. The γ-secretase complex is one of the proteases involved in processing amyloid precursor protein (APP) to amyloid beta (Aβ). Nicastrin is not catalytically active, instead, it promotes the maturation and localization of other catalytic and regulatory units of the γ-secretase complex. Nicastrin has alsp been reported to regulate neprilysin, which is the enzyme involved in the degradation of Aβ fragment.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to the C-terminal 18 a.a. of mouse Nicastrin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

In human, the antibody also detects the deglycosylated nicastrin, which is observed at ~ 78kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Esselens C, et al. 2004. J. Cell Biol. 166(7):1041. (WB)
RRID
AB_2728591 (BioLegend Cat. No. 852301)
AB_2728592 (BioLegend Cat. No. 852302)

Antigen Details

Structure
Deglycosylated nicastrin is a 709 amino acid protein with a molecular mass of ~78 kD. Higher molecular weight band of ~110 kD corresponding to glycosylated nicastrin is observed by WB.
Distribution

Tissue Distribution: Ubiquitously expressed.
Cellular: Distribution: Plasma Membrane, endoplasmic reticulum, lysosome, golgi apparatus, and cytoskeleton.

Function
Nicastrin is an essential component of the γ-secretase complex that processes amyloid precursor protein to amyloid beta. It is also involved in degradation of amyloid beta fragments.
Interaction
Presenilin, APH-1, PEN-2
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family
Enzymes and Regulators, Secretases
Antigen References
  1. Yang D, et al. 2002. J. Biol. Chem. 31:28135.
  2. Johnson DS, et al. 2017. Cold Spring Harb Perspect Med. 10.1101/cshperspect.a024067.
  3. Zhang X, et al. 2014. Front Cell Neurosci. 10.3389/fncel.2014.00427.
Gene ID
23385 View all products for this Gene ID
UniProt
View information about Nicastrin on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/29/2018

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